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Detection of AU1-tagged protein in 200, 100, and 50ng of E. coli lysate containing tagged fusion protein with #18816 diluted 1:5,000. - -
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Antibody DetailsProduct DetailsHost Species Goat Immunogen AU1 (DTYRYI) conjugated to KLH. Product Concentration 1.0 mg/ml Formulation PBS (PBS), pH 7.2, 0.09% sodium azide. State of Matter Liquid Product Preparation Purified by peptide affinity chromatography Storage and Handling This antibody is stable for at least one (1) year at 2-8°C. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: use at a dilution of 1:1,000-1:20,000
ELISA: for detection, use at a dilution of 1:1,000-1:30,000; for coating use at 1:100-1:500 Immunocytochemistry: use at a dilution of 1:100-1:400. Immunoprecipitation: use at 1-4ug/mg lysate. These are recommended dilutions. End users should determine optimal dilutions for their applications. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Goat Polyclonal Antibody specific to AU1 Background AU1 is an epitope tag often constructed on the N- or C- terminus of a protein to allow for detection and analysis of the protein. The AU1 sequence represents the amino acid sequence DTYRYI derived from the major capsid protein of bovine papillomavirus-1 (BPV-1). Antigen DetailsResearch Area Epitope Tag References & CitationsTechnical Protocols ICC   |
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
