Anti-AU1 [Polyclonal]

Anti-AU1 [Polyclonal]

Product No.: 18816

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Target
AU1
Product Type
Polyclonal
Applications
ELISA
,
ICC
,
IP
,
WB

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Select Product Size

Data

Detection of AU1-tagged protein in 200, 100, and 50ng of E. coli lysate containing tagged fusion protein with #18816 diluted 1:5,000.
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Antibody Details

Product Details

Host Species
Goat
Immunogen
AU1 (DTYRYI) conjugated to KLH.
Product Concentration
1.0 mg/ml
Formulation
PBS (PBS), pH 7.2, 0.09% sodium azide.
State of Matter
Liquid
Product Preparation
Purified by peptide affinity chromatography
Storage and Handling
This antibody is stable for at least one (1) year at 2-8°C.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
Immunoblotting: use at a dilution of 1:1,000-1:20,000

ELISA: for detection, use at a dilution of 1:1,000-1:30,000; for coating use at 1:100-1:500

Immunocytochemistry: use at a dilution of 1:100-1:400.

Immunoprecipitation: use at 1-4ug/mg lysate.

These are recommended dilutions.

End users should determine optimal dilutions for their applications.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Goat Polyclonal Antibody specific to AU1
Background
AU1 is an epitope tag often constructed on the N- or C- terminus of a protein to allow for detection and analysis of the protein. The AU1 sequence represents the amino acid sequence DTYRYI derived from the major capsid protein of bovine papillomavirus-1 (BPV-1).

Antigen Details

Research Area
Epitope Tags

References & Citations

Indirect Elisa Protocol
ICC
Immunoprecipitation Protocol
General Western Blot Protocol
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.