Anti-AU1 Antibody (18818)

Anti-AU1 Antibody (18818)

Product No.: 18818

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Target
AU1
Product Type
Polyclonal
Isotype
Whole IgG
Applications
ELISA
,
ICC
,
IP
,
WB

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Select Product Size

Data

<b>Detection of AU1-tagged Protein by western blot.</b> <i>Samples:</i> 200Detection of AU1-tagged protein in 200, 100, and 50ng of E. coli lysate containing tagged fusion protein with #18818 diluted1:25,000.
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Antibody Details

Product Details

Host Species
Rabbit
Immunogen
AU1 (DTYRYI) conjugated to KLH
Product Concentration
1.0 mg/ml
Formulation
Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
State of Matter
Liquid
Product Preparation
Purified by peptide immuno-affinity chromatography
Storage and Handling
This antibody is stable for at least one (1) year at 2-8°C.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
Immunoblotting: use at a dilution of 1:1,000-1:20,000.

Immunocytochemistry: use at a dilution of 1:100-1:400.

ELISA: for detection use at a dilution of 1:1,000-1:30,000; for coating use at a dilution of 1:100- 1:500.

Immunoprecipitation: use at 1-4ug/mg lysate

These are recommended dilutions.

Endusers should determine optimal dilutions for their applications.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rabbit Polyclonal Antibody specific to AU1.
Background
AU1 is an epitope tag often constructed on the N- or C- terminus of a protein to allow for detection and analysis of the protein. The AU1 sequence represents the amino acid sequence DTYRYI derived from the major capsid protein of bovine papillomavirus-1 (BPV-1).

Antigen Details

Research Area
Epitope Tags

References & Citations

Indirect Elisa Protocol
ICC
Immunoprecipitation Protocol
General Western Blot Protocol
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.