Anti-Bovine PKC-Biotin

Anti-Bovine PKC-Biotin

Product No.: P108

[product_table name="All Top" skus="P108"]

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Clone
MC5
Target
Protein Kinase C
Formats AvailableView All
Product Type
Monoclonal Antibody
Isotype
IgG2a
Applications
IHC FF
,
WB

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Select Product Size
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Antibody Details

Product Details

Reactive Species
Bovine
Host Species
Mouse
Product Concentration
0.5 mg/ml
Formulation
This biotinylated antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This biotinylated antibody is stable when stored at 2-8°C. Do not freeze.
Country of Origin
USA
Shipping
Next Day 2-8°C
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Anti-Protein Kinase C (PKC) recognizes the intact (Mr 79-80 kDa) calcium-activated, phospholipid-dependent Protein Kinase C in bovine brain. The antibody strongly cross-reacts with PKC in human, mouse and rat cells.1 Anti-PKC (clone MC5) binds to an epitope at the hinge region of the enzyme, the site of phorbol-ester-induced proteolysis which is thought to down-regulate PKC activity in intact cells.1,2 Monoclonal MC5 reacts with PKC-α/ß12 isoforms.
Background
Anti-PKC-Biotin (clone MC5) may be used to block both proteolysis and down-regulation of PKC activity. The antibody reacts with the intact molecule and may be used to localize PKC activity and to study the role of proteolysis in the activation of the Protein Kinase C pathway.

Antigen Details

Research Area
Apoptosis
.
Cell Biology
.
Immunology

References & Citations

1. Young, S. et al. (1988) Eur. J. Biochem. 172:247
2. Young, S. et al. (1987) Biochem. J. 244:775
IHC FF
General Western Blot Protocol

Formats Available

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Products are for research use only. Not for use in diagnostic or therapeutic procedures.