Data
Immunoblotting: Detection of c-myc-tagged protein in 200, 200, or 50ng of E. coli lysate containing tagged fusion protein with #18826 diluted 1:25,000. 
Immunofluorescence: Detection of c-myc-tagged plakoglobin (gammacatenin) on transiently transfected human microvascular endothelial cells with #18826 at 1ug/ml. - -
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Antibody DetailsProduct DetailsHost Species Rabbit Immunogen Amino acids 410-419 (EQKLISEEDL) of human myc conjugated to KLH. Product Concentration 1.0 mg/ml Formulation PBS, pH 7.2, 0.09% sodium azide. State of Matter Liquid Product Preparation Purified by peptide affinity chromatography Storage and Handling This antibody is stable for at least one (1) year at 2-8°C. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: use at a dilution of 1:1,000- 1:25,000
Immunocytochemistry: use at a dilution of 1:100-1:400. Immunofluorescence: use at a dilution of 1:100-1:400 ELISA: for detection use at a dilution of 1:1,000-1:30,000; for coating use at a dilution of 1:100-1:500. These are recommended dilutions. End users should determine optimal dilutions for their applications. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Rabbit Polyclonal Antibody specific to c-myc. Background c-myc is an epitope tag often constructed on the N- or C-terminus of a protein to allow for detection and analysis of the protein. The peptide sequence of the myc tag is EQKLISEEDL (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu). The c-myc tag is approximately 1202 Daltons in atomic mass and includes 10 amino acids. Antigen DetailsResearch Area Epitope Tag References & CitationsTechnical Protocols ICC   |
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