Anti-CRISPR/Cas9 (Clone 7A9-3A3)- Biotin

Anti-CRISPR/Cas9 (Clone 7A9-3A3)- Biotin

Product No.: C3456

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Clone
7A9-3A3
Target
CRISPR/Cas9
Formats AvailableView All
Product Type
Hybridoma Monoclonal Antibody
Alternate Names
CRISPR associated protein 9, SpyCas9
Isotype
Mouse IgG1 κ
Applications
FC
,
IF
,
IHC
,
IP
,
WB

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Antibody Details

Product Details

Reactive Species
S. Pyogenes
Host Species
Mouse
Immunogen
Recombinant S. pyogenes Cas9 Protein (N-terminal 250 amino acids) expressed in E. coli.
Product Concentration
0.5 mg/ml
Formulation
This Biotinylated antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
State of Matter
Liquid
Storage and Handling
This biotinylated antibody is stable when stored at 2-8°C. Do not freeze.
Regulatory Status
Research Use Only
Country of Origin
USA
Shipping
2 – 8° C Wet Ice
Additional Applications Reported In Literature ?
IHC
IP
IF
WB
FC
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Description

Specificity
7A9-3A3 activity is directed against Cas9 and dCas9
Background
The CRISPR/Cas9 system is an RNA-guided endonuclease system used for genome editing that was first identified as an adaptive immune system in bacteria and archaea1. To operate, a single guide RNA is responsible for DNA site targeting, while Cas9 cleaves the DNA at the target sites. Cas9 contains RuvC and HNH nuclease domains, which are responsible for cleaving the target complementary and noncomplementary DNA strands. Cas9 is directed to the specific DNA targets by the single guide RNA. Any DNA sequence that contains the N20-NGG motif can be recognized as a target site.

The CRISPR/Cas9 system has been engineered to allow genome editing in a variety of organisms beyond bacteria1. Codon-optimized Cas9 with an appropriate nuclear localization signal has been used in yeast, roundworm, silkworm, Drosophila, zebrafish, frog, rabbit, human, mouse, rat, pig, monkey, and plants. A single gene or multiple genes (e.g., at least four genes in rat, mouse, and zebrafish) can be disrupted by providing the appropriate single guide RNAs and programming the DNA-cleaving activity of Cas9. Large-fragment deletion, inversion, and reporter gene insertion can also be performed. Additionally, methylation effect and chromatin states can be studied using ‘dead’ Cas9, which is a fusion of a non-functional Cas9 with an effector domain. In mouse1, 2, 3 and humans, the CRISPR/Cas9 system has been used to correct genetic diseases (e.g., cystic fibrosis1, sickle cell disease4, and β-thalassemia) and also has applications for cancer treatment5.
Antigen Distribution
Cas9 is found naturally in the prokaryote Streptococcus pyogenes and can be introduced into various species for genome editing.
NCBI Gene Bank ID
UniProt.org
Research Area
Cell Biology
.
Neuroscience

References & Citations

1. Ma Y, Zhang L, Huang X. FEBS J. 281(23):5186-5193. 2014.
2. Savić N, Schwank G. Transl Res. 168:15-21. 2016.
3. Park H, Oh J, Shim G, et al. Nat Neurosci. 22(4):524-528. 2019.
4. Frangoul H, Altshuler D, Cappellini MD, et al. N Engl J Med. 384(3):252-260. 2021.
5. Wang SW, Gao C, Zheng YM, et al. Mol Cancer.21(1):57. 2022.
6. Lin S, Ewen-Campen B, Ni X, et al. Genetics. 201(2):433-442. 2015.
7. Howden SE, McColl B, Glaser A, et al. Stem Cell Reports. 7(3):508-517. 2016.
8. Kim S, Bae T, Hwang J, et al. Genome Biol. 18(1):218. 2017.
9. Lattanzi A, Duguez S, Moiani A, et al. Mol Ther Nucleic Acids. 7:11-19. 2017.
10. Maji B, Moore CL, Zetsche B, et al. Nat Chem Biol. 13(1):9-11. 2017.
Flow Cytometry
IF
IHC
Immunoprecipitation Protocol
General Western Blot Protocol

Certificate of Analysis

Formats Available

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Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.