Anti-DNA Ligase I [Clone 10H5]
Anti-DNA Ligase I [Clone 10H5]
Product No.: 56219
Data
![Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DNA ligase I antibody [10H5] (56219) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](https://www.leinco.com/wp-content/uploads/2025/01/qed-bioscience-anti-dna-ligase-i-antibody-56219-1.jpg)
Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DNA ligase I antibody [10H5] (56219) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.![DNA ligase I antibody [10H5] detects DNA ligase I protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DNA ligase I stained by DNA ligase I antibody [10H5] (56219) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar= 10 um.](https://www.leinco.com/wp-content/uploads/2025/01/qed-bioscience-anti-dna-ligase-i-antibody-56219-2.jpg)
DNA ligase I antibody [10H5] detects DNA ligase I protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DNA ligase I stained by DNA ligase I antibody [10H5] (56219) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar= 10 um.![DNA ligase I antibody [10H5] detects DNA ligase I protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. DNA ligase I stained by DNA ligase I antibody [10H5] (56219) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.leinco.com/wp-content/uploads/2025/01/qed-bioscience-anti-dna-ligase-i-antibody-56219-3.jpg)
DNA ligase I antibody [10H5] detects DNA ligase I protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
DNA ligase I stained by DNA ligase I antibody [10H5] (56219) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min - -
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Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Immunogen Recombinant human DNA Ligase I (aa 1-919) purified from baculovirus infected Sf9 cells. Product Concentration Lot Specific Formulation PBS, pH 7.4 State of Matter Liquid Product Preparation Purified by Protein G affinity chromatography Storage and Handling This antibody is stable for at least one (1) year at -70°C. Avoid multiple freeze- thaw cycles. Regulatory Status Research Use Only Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: use at 1-5 ug/mL.
Immunoprecipitation: use at 1-5 ug/mL. Positive controls: MCF-7 cells or recombinant protein. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity This antibody recognizes human DNA Ligase I (125 kD), one of the four ligase proteins found in mammalian cells that are required for the joining of DNA strand breaks that occur during DNA replication, repair, and recombination. Function DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair. NCBI Gene Bank ID UniProt.org Research Area Cancer Research References & CitationsTechnical ProtocolsICC IF IHC FFPE   Certificate of Analysis |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
