Anti-DNA Ligase I Antibody (56219)

Product No.: 56219


Clone 10H5 Target DNA Ligase I Formats AvailableView All Product Type Monoclonal Alternate Names EC 6.5.1.1, DNA ligase I, Polydeoxyribonucleotide synthase [ATP] 1 Isotype Mouse IgG1 Applications WB,ICC/IF,IHC-P,IP


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Data

Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DNA ligase I antibody [10H5] (56219) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

DNA ligase I antibody [10H5] detects DNA ligase I protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DNA ligase I stained by DNA ligase I antibody [10H5] (56219) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar= 10 um.

DNA ligase I antibody [10H5] detects DNA ligase I protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. DNA ligase I stained by DNA ligase I antibody [10H5] (56219) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min


Antibody Details Product Details Reactive Species Human Host Species Mouse Immunogen Recombinant human DNA Ligase I (aa 1-919) purified from baculovirus infected Sf9 cells. Product Concentration Lot Specific Formulation PBS, pH 7.4 State of Matter Liquid Product Preparation Purified by Protein G affinity chromatography Storage and Handling This antibody is stable for at least one (1) year at -70°C. Avoid multiple freeze- thaw cycles. Regulatory Status Research Use Only Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: use at 1-5 ug/mL. Immunoprecipitation: use at 1-5 ug/mL. Positive controls: MCF-7 cells or recombinant protein. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Specificity This antibody recognizes human DNA Ligase I (125 kD), one of the four ligase proteins found in mammalian cells that are required for the joining of DNA strand breaks that occur during DNA replication, repair, and recombination. Function DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair. NCBI Gene Bank ID LIG1 UniProt.org P18858 Research Area Cancer Research References & Citations View product citations for antibody PRODUCT_CODE on CiteAb Request COA

Antibody Details

Product Details

Reactive Species

Human

Host Species

Mouse

Immunogen

Recombinant human DNA Ligase I (aa 1-919) purified from baculovirus infected Sf9 cells.

Product Concentration

Lot Specific

Formulation

PBS, pH 7.4

State of Matter

Liquid

Product Preparation

Purified by Protein G affinity chromatography

Storage and Handling

This antibody is stable for at least one (1) year at -70°C. Avoid multiple freeze- thaw cycles.

Regulatory Status

Research Use Only

Country of Origin

USA

Shipping

Next Day 2-8°C

Applications and Recommended Usage?
Quality Tested by Leinco

Immunoblotting: use at 1-5 ug/mL.

Immunoprecipitation: use at 1-5 ug/mL.

Positive controls: MCF-7 cells or recombinant protein.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

This antibody recognizes human DNA Ligase I (125 kD), one of the four ligase proteins found in mammalian cells that are required for the joining of DNA strand breaks that occur during DNA replication, repair, and recombination.

Function

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.

NCBI Gene Bank ID

LIG1

UniProt.org

P18858

Research Area

Cancer Research