Anti-DNA Ligase III [Clone 1F3]
Anti-DNA Ligase III [Clone 1F3]
Product No.: 56250
Data
DNA ligase III antibody detects DNA ligase III protein by western blot analysis. Whole cell extracts (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase III antibody (56250) diluted by 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.![DNA ligase III detects DNA ligase III antibody [1F3] protein by western blot analysis. A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract 7.5% SDS-PAGE DNA ligase III (56250) dilution: 1:500 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](https://www.leinco.com/wp-content/uploads/2025/01/qed-bioscience-anti-dna-ligase-iii-antibody-56250-2.jpg)
DNA ligase III detects DNA ligase III antibody [1F3] protein by western blot analysis.
A. 30 ug 293T whole cell lysate/extract
B. 30 ug A431 whole cell lysate/extract
C. 30 ug HeLa whole cell lysate/extract
D. 30 ug HepG2 whole cell lysate/extract
7.5% SDS-PAGE
DNA ligase III (56250) dilution: 1:500
The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody. - -
- -
Antibody DetailsProduct DetailsReactive Species Chicken ⋅ Human ⋅ Mouse Host Species Mouse Immunogen Full-length recombinant human DNA Ligase III-b. Product Concentration 1.0 mg/ml Formulation Hybridoma culture supernatant State of Matter Liquid Product Preparation Purified by Protein G affinity chromatography Storage and Handling This antibody is stable for at least one year at -20°C. Store in appropriate aliquots to avoid multiple freeze-thaw cycles. Regulatory Status Research Use Only Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: use at 0.5-2ug/mL. Predicted molecular weight ~100kDa.
These are recommended concentrations. Endusers should determine optimal concentrations for their applications. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity This antibody recognizes human, mouse, and chicken DNA Ligase III. Cellular localization is nuclear and mitochondrial. Background There are four distinct ligase proteins found in mammalian cells. These DNA ligases are required for joining DNA strand breaks that occur during DNA replication, repair and recombination. DNA Ligase III is a 100kDa (a-form) protein in somatic tissues, is ubiquitously expressed, and complexes with XRCC-1 and a 97kDa (§-form) protein that is found in testis. DNA Ligase III is involved in rejoining DNA single-strand breaks and in base excision repair pathway. Function Isoform 3 functions as heterodimer with DNA-repair protein XRCC1 in the nucleus and can correct defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents. Isoform 1 is targeted to mitochondria, where it functions as DNA ligase in mitochondrial base-excision DNA repair (PubMed:10207110, PubMed:24674627). {PubMed:10207110, PubMed:24674627}. NCBI Gene Bank ID UniProt.org Research Area Cancer Research References & CitationsTechnical ProtocolsB ICC IF   Certificate of Analysis |
Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
