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![E2F1 antibody [17E2] detects E2F1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. E2F1 stained by E2F1 antibody [17E2] (3332) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.leinco.com/wp-content/uploads/2025/01/qed-bioscience-anti-e2f-1-antibody-3332-2.jpg)
E2F1 antibody [17E2] detects E2F1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
E2F1 stained by E2F1 antibody [17E2] (3332) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min![Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with E2F1 antibody [17E2] (3332) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](https://www.leinco.com/wp-content/uploads/2025/01/qed-bioscience-anti-e2f-1-antibody-3332-1.jpg)
Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with E2F1 antibody [17E2] (3332) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody. - -
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Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Immunogen GST fusion protein expressed in E. coli corresponding to aa 162-476 of human E2F-1. Product Concentration Lot Specific Formulation PBS, pH 7.4 State of Matter Liquid Product Preparation Purified by Protein G affinity chromatography Storage and Handling This antibody is stable for at least one (1) year at -70°C. Avoid multiple freeze- thaw cycles. Regulatory Status Research Use Only Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: use at 1-5 ug/mL.
Immunoprecipitation: use at 1-5 ug/mL. Positive controls: E2F-1 transfected Soas2 cells and recombinant fusion protein. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity This antibody recognizes an epitope within aa 332-390 of human E2F-1. Function Transcription activator that binds DNA cooperatively with DP proteins through the E2 rPubMed:10675335, PubMed:12717439, PubMed:17050006, PubMed:17704056, PubMed:18625225, PubMed:20040599, PubMed:20176812, PubMed:28992046, PubMed:8170954}. NCBI Gene Bank ID UniProt.org Research Area Cancer Research References & CitationsTechnical ProtocolsIHC FFPE   Certificate of Analysis |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
