Anti-E2F-1 Antibody (3333)

Product No.: 3333


Clone 6B5 Target E2F-1 Formats AvailableView All Product Type Monoclonal Alternate Names E2F-1, PBR3, Retinoblastoma-associated protein 1, RBAP-1, Retinoblastoma-binding protein 3, RBBP-3, pRB-binding protein E2F-1 Isotype Mouse IgG1 Applications WB,IP


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Data

Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with E2F1 antibody [6B5] (3333) diluted at 1:500. The signal was developed with Trident ECL plus-Enhanced.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with E2F1 antibody [6B5] (3333) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.


Antibody Details Product Details Reactive Species Human Host Species Mouse Immunogen GST fusion protein expressed in E. coli corresponding to aa 162-476 of human E2F-1. Product Concentration Lot Specific Formulation PBS, pH 7.4 State of Matter Liquid Product Preparation Purified by Protein G affinity chromatography Storage and Handling This antibody is stable for at least one (1) year at -70°C. Avoid multiple freeze- thaw cycles. Regulatory Status Research Use Only Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: use at 1-5 ug/mL. Immunoprecipitation: use at 1-5 ug/mL. Positive controls: E2F-1 transfected Soas2 cells and recombinant fusion protein. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Specificity This antibody recognizes an epitope within aa 223-322 of human E2F-1. Function Transcription activator that binds DNA cooperatively with DP proteins through the E2 rPubMed:10675335, PubMed:12717439, PubMed:17050006, PubMed:17704056, PubMed:18625225, PubMed:20040599, PubMed:20176812, PubMed:28992046, PubMed:8170954}. NCBI Gene Bank ID E2F1 UniProt.org Q01094 Research Area Cancer Research References & Citations View product citations for antibody PRODUCT_CODE on CiteAb Request COA

Antibody Details

Product Details

Reactive Species

Human

Host Species

Mouse

Immunogen

GST fusion protein expressed in E. coli corresponding to aa 162-476 of human E2F-1.

Product Concentration

Lot Specific

Formulation

PBS, pH 7.4

State of Matter

Liquid

Product Preparation

Purified by Protein G affinity chromatography

Storage and Handling

This antibody is stable for at least one (1) year at -70°C. Avoid multiple freeze- thaw cycles.

Regulatory Status

Research Use Only

Country of Origin

USA

Shipping

Next Day 2-8°C

Applications and Recommended Usage?
Quality Tested by Leinco

Immunoblotting: use at 1-5 ug/mL.

Immunoprecipitation: use at 1-5 ug/mL.

Positive controls: E2F-1 transfected Soas2 cells and recombinant fusion protein.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

This antibody recognizes an epitope within aa 223-322 of human E2F-1.

Function

Transcription activator that binds DNA cooperatively with DP proteins through the E2 rPubMed:10675335, PubMed:12717439, PubMed:17050006, PubMed:17704056, PubMed:18625225, PubMed:20040599, PubMed:20176812, PubMed:28992046, PubMed:8170954}.

NCBI Gene Bank ID

E2F1

UniProt.org

Q01094

Research Area

Cancer Research