Sample (30 ug of whole cell lysate)
A: 293T
10% SDS PAGE
3334 diluted at 1:1000
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Antibody Details
Product Details
Reactive Species
Human
Host Species
Mouse
Immunogen
GST fusion protein expressed in E. coli corresponding to aa 162-476 of human E2F-1.
Product Concentration
Lot Specific
Formulation
PBS, pH 7.4
State of Matter
Liquid
Product Preparation
Purified by Protein G affinity chromatography
Storage and Handling
This antibody is stable for at least one (1) year at -70°C. Avoid multiple freeze- thaw cycles.
Regulatory Status
Research Use Only
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage? Quality Tested by Leinco
Immunoblotting: use at 1-5 ug/mL.
Immunoprecipitation: use at 1-5 ug/mL.
Positive controls: E2F-1 transfected Soas2 cells and recombinant fusion protein.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Description
Specificity
This antibody recognizes an epitope within aa 244-264 of human E2F-1.
Function
Transcription activator that binds DNA cooperatively with DP proteins through the E2 rPubMed:10675335, PubMed:12717439, PubMed:17050006, PubMed:17704056, PubMed:18625225, PubMed:20040599, PubMed:20176812, PubMed:28992046, PubMed:8170954}.