Anti-Human Fibronectin Antibody (42042)

Anti-Human Fibronectin Antibody (42042)

Product No.: 42042

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Clone
FN30-8
Target
Fibronectin
Formats AvailableView All
Product Type
Monoclonal
Alternate Names
FN, Cold-insoluble globulin, CIG [Cleaved into: Anastellin; Ugl-Y1; Ugl-Y2; Ugl-Y3]
Isotype
IgG1
Applications
ELISA
,
WB
,
Inhibition

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Select Product Size
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Antibody Details

Product Details

Reactivity Species
Human
Host Species
Mouse
Immunogen
Human plasma fibronectin
Formulation
PBS, pH 7.4, 1% BSA, lyophilized.
State of Matter
Lyophilized
Product Preparation
Purified by immunoaffinity chromatography
Storage and Handling
The stock solution (2. 0mg/ml) can be stored in aliquots at -20°C for 1 year or can be stored at 4°C for 6 months after adding 0.1% sodium azide. Prepare dilutions of stock solution immediately before use. Do not store diluted stock solution. Avoid repeated freeze-thaw cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
ELISA: 1-10ug/ml with immobilized antigen

Western Blot: 1-10ug/ml, reducing or non-reducing conditions

Cell Adhesion Inhibition Assay: 0.1-1.0ug/ml on 10ug/ml fibronectin-coated microwells inhibits adhesion of trypsinized NRK cells.

Immunohistochemistry: 1-10ug/ml, paraffin-embedded or frozen tissue sections with Proteinase K treatment for antigen retrieval.

These are recommended concentrations.

End users should determine optimal concentrations for their applications.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Mouse Monoclonal Antibody specific to the cell-binding domain of human fibronectin and can inhibit cell adhesion.
Background
Fibronectin is a 440kDa glycoprotein of the extracellular matrix that binds to integrins, collagen, fibrin, and heparan sulfate proteoglycans. Fibronectin plays a major role in cell adhesion, growth, migration, and differentiation, and it is important for processes such as wound healing and embryonic development. Altered fibronectin expression, degradation, and organization has been associated with a number of pathologies, including cancer and fibrosis.

Antigen Details

Function
Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin (PubMed:3024962, PubMed:3900070, PubMed:3593230, PubMed:7989369). Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape (PubMed:3024962, PubMed:3900070, PubMed:3593230, PubMed:7989369). Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization (By similarity). Participates in the regulation of type I collagen deposition by osteoblasts (By similarity). Acts as a ligand for the LILRB4 receptor, inhibiting FCGR1A/CD64-mediated monocyte activation (PubMed:34089617). {UniProtKB:P11276, PubMed:3024962, PubMed:34089617, PubMed:3593230, PubMed:3900070, PubMed:7989369}.; [Anastellin]: Binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling. {PubMed:11209058, PubMed:15665290, PubMed:19379667, PubMed:8114919}.
NCBI Gene Bank ID
UniProt.org
Research Area
Cell Adhesion

References & Citations

Indirect Elisa Protocol
General Western Blot Protocol
Inhibition

Formats Available

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Prod No.
Description
42042
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.