Anti-Glu-Glu Antibody (18836)

Anti-Glu-Glu Antibody (18836)

Product No.: 18836

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Target
Glu-Glu
Product Type
Polyclonal
Applications
ELISA
,
ICC
,
IP
,
WB

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Select Product Size

Data

Immunoblotting: Detection of Glu-Glu-tagged protein in 200, 100, and 50ng of E. coli lysate containing tagged fusion protein with #18836 diluted 1:5,000.
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Antibody Details

Product Details

Host Species
Goat
Immunogen
Synthetic peptide corresponding to aa 314-319 (EYMPME) of middle T antigen of mouse polyomavirus conjugated to KLH.
Product Concentration
1.0 mg/ml
Formulation
Phosphate Buffered Saline (PBS), 0.09% sodium azide.
State of Matter
Liquid
Product Preparation
Purified by peptide affinity chromatography
Storage and Handling
This antibody is stable for at least one (1) year at 2-8°C.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
Immunoblotting: use at a dilution of 1:1,000- 1:20,000

Immunocytochemistry: use at a dilution of 1:100-1:400.

Immunoprecipitation: use 1-4ug/mg lysate

ELISA: for detection use at a dilution of 1:1,000-1:30,000; for coating use at a dilution of 1:100-1:500.

These are recommended dilutions.

End users should determine optimal dilutions for their applications.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Goat Polyclonal Antibody specific to Glu-Glu.
Background
The Glu-Glu tag represents the peptide sequence EYMPME which is derived from the middle T antigen of polyoma virus. Given the small size of this peptide, it is unlikely that modifying proteins with it will affect protein structure or function.

Antigen Details

Research Area
Epitope Tags

References & Citations

Indirect Elisa Protocol
ICC
Immunoprecipitation Protocol
General Western Blot Protocol
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.