Data
Immunoblotting:Detection of Glu-Glu-tagged protein in E. coli lysate containing 200, 100, and 50ng of tagged protein with #18838 diluted 1:25,000. - -
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Antibody DetailsProduct DetailsHost Species Rabbit Immunogen Synthetic peptide corresponding to aa 314-319 (EYMPME) of middle T antigen of mouse polyomavirus conjugated to KLH. Product Concentration 1.0 mg/ml Formulation Phosphate Buffered Saline (PBS), 0.09% sodium azide. State of Matter Liquid Product Preparation Purified by peptide affinity chromatography Storage and Handling This antibody is stable for at least one (1) year at 2-8°C. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: use at a dilution of 1:1,000- 1:20,000
Immunocytochemistry: use at a dilution of 1:100-1:400. Immunoprecipitation: use 1-4ug/mg lysate ELISA: for detection use at a dilution of 1:1,000-1:30,000; for coating use at a dilution of 1:100-1:500. These are recommended dilutions. End users should determine optimal dilutions for their applications. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Rabbit Polyclonal Antibody specific to Glu-Glu. Background The Glu-Glu tag represents the peptide sequence EYMPME which is derived from the middle T antigen of mouse polyoma virus. Given the small size of this peptide, it is unlikely that modifying proteins with it will affect protein structure or function. Antigen DetailsResearch Area Epitope Tag References & CitationsTechnical Protocols ICC   |
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
