Anti-Glutathione-S-Transferase (GST) [Clone 3G10]
Anti-Glutathione-S-Transferase (GST) [Clone 3G10]
Product No.: 18803
Data
![GST tag antibody [3G10] detects GST tag protein by Western blot analysis. A. Non-induced cell lysate from E. coli expressing GST-tagged proteins B. IPTG-induced cell lysate from E. coli expressing GST-tagged proteins 10 % SDS-PAGE GST tag antibody [3G10] (18803) dilution: 1:10000](https://www.leinco.com/wp-content/uploads/2025/01/qed-bioscience-anti-glutathione-s-transferase-gst-antibody-18803-1.jpg)
GST tag antibody [3G10] detects GST tag protein by Western blot analysis.
A. Non-induced cell lysate from E. coli expressing GST-tagged proteins
B. IPTG-induced cell lysate from E. coli expressing GST-tagged proteins
10 % SDS-PAGE
GST tag antibody [3G10] (18803) dilution: 1:10000![GST tag antibody [3G10] detects GST tag protein by Western blot analysis. A. 2 ug His-recombinant protein B. 2 ug GST-recombinant protein C. 1 ug GST-recombinant protein D. 0.5 ug GST-recombinant protein 10 % SDS-PAGE GST tag antibody [3G10] (18803) dilution: 1:5000](https://www.leinco.com/wp-content/uploads/2025/01/qed-bioscience-anti-glutathione-s-transferase-gst-antibody-18803-2.jpg)
GST tag antibody [3G10] detects GST tag protein by Western blot analysis.
A. 2 ug His-recombinant protein
B. 2 ug GST-recombinant protein
C. 1 ug GST-recombinant protein
D. 0.5 ug GST-recombinant protein
10 % SDS-PAGE
GST tag antibody [3G10] (18803) dilution: 1:5000 - -
- -
Antibody DetailsProduct DetailsReactive Species Glutathione-S-Transferase (Gst) Host Species Mouse Immunogen GST protein of Schistosoma japonicum expressed in E. coli. Product Concentration 1.0 mg/ml Formulation PBS, pH 7.4. State of Matter Liquid Product Preparation Purified by Protein G affinity chromatography Storage and Handling This antibody is stable for at least one (1) year at -20°C. Store product in appropriate aliquots to
avoid multiple freeze-thaw cycles. Regulatory Status Research Use Only Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: use at 0.2-2ug/mL.
These are recommended concentrations. Endusers should determine optimal concentrations for their applications. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity This antibody recognizes GST. Background A GST-tag is often used to separate and purify proteins that contain the GST-fusion protein. The tag is 220 amino acids which, compared to tags such as the myc-tag, is quite large. It can be fused to either the N-terminus or C-terminus of a protein. Many commercially available sources of GST-tagged plasmids include a thrombin domain for cleavage of the GST tag during protein purification. Function Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.; GST isoenzymes appear to play a central role in the parasite detoxification system. Other functions are also suspected including a role in increasing the solubility of haematin in the parasite gut. UniProt.org Research Area Epitope Tag References & Citations1. Mosser AG et al. 2002 J Infect Dis 185: 734. 2. Mosser AG et al. 2005 Am J Respir Crit Care Med 171: 645. 3. Jurgeit A et a. 2010 Virology J 7: 264. 4. Chattoraj SS et al. 2011 Infect Immun 79: 4131. Technical Protocols  Certificate of Analysis |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
