Anti-HDEL Antibody (11077)
Anti-HDEL Antibody (11077)
Product No.: 11077
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Clone 2-E7 Target HDEL Formats AvailableView All Product Type Monoclonal Isotype Mouse IgG2b Applications WB , ICC/IF |
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Antibody DetailsProduct DetailsReactivity Species Drosophila, Yeast, Plants Host Species Mouse Immunogen Synthetic peptide corresponding to the C-terminus of yeast Bip. Product Concentration Lot Specific Formulation PBS, pH 7.4, 50% glycerol, and 0.09% sodium azide. State of Matter Liquid Product Preparation Purified by Protein G affinity chromatography Storage and Handling This antibody is stable for at least one (1) year at -20°C. Regulatory Status For in vitro investigational use only. Not for
use in therapeutic or diagnostic procedures. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: use at 0.5-1ug/ml. A band of ~70 kDa is detected.
Immunofluorescence: use at 0.2-2ug/ml. These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: S. cerevisiae lysate. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity This antibody recognizes a ~70 kDa protein corresponding to the predicted molecular mass of HDEL in Drosophila, yeast, and various plants (barnyard grass, beet, cotton, mung bean, sorghum, and wheat). Background The Hsp70 family includes four highly conserved proteins (Hsp70, Hsc70, Grp75, and Grp78) which function as molecular chaperones, in translocation of polypeptides across cell membranes and to cell nuclei, and in proper folding of nascent polypeptide chains. Sorting of proteins by the Hsp70 family relies on a C-terminal tetrapeptide signal, usually KDEL in animal cells, and HDEL in S. cerevisia. Antigen DetailsResearch Area Heat Shock & Stress Proteins References & CitationsNapier RM et al. 1992 J Cell Science 102: 261-271. Technical Protocols ICC/IF |
Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
