Anti-Heme Oxygenase-1 Antibody (13062)

His-tagged rat heme-oxygenase-1 (HO-1)

PBS, pH 7.4, 0.1% sodium azide, 50% glycerol.

Liquid

Purified by Protein G affinity chromatography

This antibody is stable for at least one (1) year at -20°C. Avoid repeated freeze-thaw cycles.

Research Use Only

Next Day 2-8°C

This antibody recognizes human, mouse, and rat HO-1. It does not cross-react with HO-2.

Heme-oxygenase is an enzyme that catalyzes heme catabolism to yield biliverdin, iron, and carbon monoxide (CO). There are three isoforms of heme-oxygenase: HO-1, HO-2, and HO-3. HO-1 and HO-2 have been identified as the two major isoforms in mammals. HO-1, also known as heat shock protein 32 (Hsp32) is induced by most oxidative stress inducers, cytokines, inflammatory agents, and heat shock. HO-1 deficiency appears to cause reduced stress defense, a pro-inflammatory tendency, susceptibility to atherosclerotic lesion formation, endothelial cell injury, and growth retardation. Therefore, up-regulation of HO-1 is one of the major defense mechanisms against oxidative stress.

Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Exhibits cytoprotective effects since excess of free heme sensitizes cells to undergo apoptosis.