Anti-High MW Kininogen Heavy Chain Antibody (20002)
Anti-High MW Kininogen Heavy Chain Antibody (20002)
Product No.: 20002
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Clone 2B5 Target High MW Kininogen Heavy Chain Formats AvailableView All Product Type Monoclonal Alternate Names α-2-thiol proteinase inhibitor, Fitzgerald factor, High molecular weight kininogen, HMWK, Williams-Fitzgerald-Flaujeac factor [Cleaved into: Kininogen-1 heavy chain; T-kinin] Isotype Mouse IgG1 Applications ELISA , WB , Coagulation Assays |
Data
A. Immunoblot with #20002 on purified HMWK (see reference below). B. HMWK Coomasie is SDS-PAGE of purified HMWK stained with Coomassie Blue - -
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Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Immunogen Purified human high molecular weight kininogen. Product Concentration Lot Specific Formulation PBS, pH 7.4. State of Matter Liquid Product Preparation Purified by Protein G affinity chromatography Storage and Handling This antibody is stable for at least one (1) year at -20°C. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco This antibody may be used in ELISA and Western blots to detect human HMWK light chain. It may also be used in coagulation assays.
Enduser should determine optimal antibody concentrations for their applications. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity This antibody recognizes the common heavy chain (~64kDa) of human HMWK and can
neutralize the inhibitory activity of HMWK on platelet calpain. Background High molecular weight kininogen (HMWK) is a circulating plasma protein which participates in the initiation of blood coagulation, and in the generation of the vasodilator bradykinin via the Kallikrein-kinin system. HMWK is inactive until it either adheres to binding proteins beneath an endothelium disrupted by injury, thereby initiating coagulation, or it binds to intact endothelial cells or platelets for functions other than coagulation. HMWK is an alpha-globulin with six functional domains. It circulates as a single-chain 626 amino acid polypeptide . The heavy chain contains domains 1, 2, and 3; the light chain, domains 5 and 6. Domain 4 links the heavy and light chains. Function (1) Kininogens are inhibitors of thiol proteases; (2) HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII; (3) HMW-kininogen inhibits the thrombin- and plasmin-induced aggregation of thrombocytes; (4) the active peptide bradykinin that is released from HMW-kininogen shows a variety of physiological effects: (4A) influence in smooth muscle contraction, (4B) induction of hypotension, (4C) natriuresis and diuresis, (4D) decrease in blood glucose level, (4E) it is a mediator of inflammation and causes (4E1) increase in vascular permeability, (4E2) stimulation of nociceptors (4E3) release of other mediators of inflammation (e.g. prostaglandins), (4F) it has a cardioprotective effect (directly via bradykinin action, indirectly via endothelium-derived relaxing factor action); (5) LMW-kininogen inhibits the aggregation of thrombocytes; (6) LMW-kininogen is in contrast to HMW-kininogen not involved in blood clotting. NCBI Gene Bank ID UniProt.org Research Area Coagulation factors References & CitationsSchmaier et al. (1987) Journal of Biological Chemistry 262: 1405-1411. Technical Protocols  Coagulation Assays Certificate of Analysis |
Formats Available
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Prod No. | Description |
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56629 | |
20002 |
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
