Anti-Human Bcl-x

Pricing & Details

Product No.B640
Protein
Bcl-x
Formats AvailableView All
Product Type
Polyclonal Antibody
Applications
IP
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
B640-25 µg
25 µg
$254.00
In stock
Max:
Min: 1
Step: 1
B640-100 µg
100 µg
$359.00
In stock
Max:
Min: 1
Step: 1

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Rabbit
Immunogen
Purified Recombinant (aa 49-68)(>98%)
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Storage and Handling
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect this polyclonal antibody can be used at a concentration of 0.3 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as and a chromogenic substrate such as PN:T343. The detection limit for is 1.5-3.0 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Simple Western: Suitable for use at concentration of 3 µg/mL
Other Applications Reported In Literature ?
Immunoprecipitation: Suitable for use at concentration of 5 µg/106 cells.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rabbit Anti-Human Bcl-x recognizes an epitope on Human Bcl-x . This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Antigen Details

Protein
PubMed
NCBI Gene Bank ID
Immunoprecipitation Protocol
General Western Blot Protocol
Prod No.
Description
B640
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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