Anti-Human BCMA

Anti-Human BCMA

Product No.: B629


Target BCMA Product Type Polyclonal Antibody Alternate Names B-Cell Maturation Antigen, TNFRSF17, BCM Applications B , CyTOF® , ELISA , FC , WB


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Antibody Details Product Details Reactive Species Human Host Species Goat Immunogen Purified Recombinant Human BCMA (>98%) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2828289 Applications and Recommended Usage? Quality Tested by Leinco ELISA: This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human BCMA/TNFRSF17 Monoclonal Antibody. This product is intended for assay development on various assay platforms requiring antibody pairs. Western Blotting: To detect Human BCMA this polyclonal antibody can be used at a concentration of 0.1-0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Human BCMA is 1.0 and 5.0 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:B-374. Flow Cytometry: Suitable for use at concentration of 0.25 µg/106 cells. Additional Applications Reported In Literature ? Blockade of Receptor-ligand Interaction: In a functional ELISA, 0.3-1.2 µg/mL of this antibody will block 50% of the binding of 500 ng/mL of Recombinant Human APRIL/TNFSF13 to immobilized Recombinant Human BCMA/TNFRSF17 Fc Chimera coated at 1 µg/mL (100 µL/well). At 15 μg/mL, this antibody will block >90% of the binding. CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Specificity Goat Anti-Human B cell maturation protein (BCMA) recognizes Human BCMA. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Antibody has ability to block receptor-ligand interaction. PubMed BCMA NCBI Gene Bank ID 608 UniProt.org More Information on Uniprot.org References & Citations View product citations for antibody PRODUCT_CODE on CiteAb Technical Protocols B CyTOF® Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Human

Host Species

Goat

Immunogen

Purified Recombinant Human BCMA (>98%)

Endotoxin Level

<0.1 EU/µg as determined by the LAL method

Formulation

This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.

State of Matter

Lyophilized

Storage and Handling

The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2828289

Applications and Recommended Usage?
Quality Tested by Leinco

ELISA: This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human BCMA/TNFRSF17 Monoclonal Antibody. This product is intended for assay development on various assay platforms requiring antibody pairs.
Western Blotting: To detect Human BCMA this polyclonal antibody can be used at a concentration of 0.1-0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Human BCMA is 1.0 and 5.0 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:B-374.
Flow Cytometry: Suitable for use at concentration of 0.25 µg/106 cells.

Additional Applications Reported In Literature ?

Blockade of Receptor-ligand Interaction: In a functional ELISA, 0.3-1.2 µg/mL of this antibody will block 50% of the binding of 500 ng/mL of Recombinant Human APRIL/TNFSF13 to immobilized Recombinant Human BCMA/TNFRSF17 Fc Chimera coated at 1 µg/mL (100 µL/well). At 15 μg/mL, this antibody will block >90% of the binding.
CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Goat Anti-Human B cell maturation protein (BCMA) recognizes Human BCMA. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Antibody has ability to block receptor-ligand interaction.

PubMed

BCMA

NCBI Gene Bank ID

608

UniProt.org

More Information on Uniprot.org