Anti-Human CD257 (BAFF) (Tabalumab)

Anti-Human CD257 (BAFF) (Tabalumab)

Product No.: LT1400

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Product No.LT1400
Clone
Tabalumab
Target
BAFF
Product Type
Biosimilar Recombinant Human Monoclonal Antibody
Alternate Names
Tabalumab, CD257, BAFF, TNFSF13b, BLYS, 1143503-67-6
Isotype
Human IgG1κ
Applications
ELISA
,
FA
,
FC
,
IF
,
IP
,
N
,
WB

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Select Product Size
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Antibody Details

Product Details

Reactive Species
Cynomolgus Monkey
Human
Rabbit
Host Species
Human
Expression Host
HEK-293 Cells
FC Effector Activity
Active
Immunogen
Original antibody was raised against soluble human BAFF.
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
< 1.0 EU/mg as determined by the LAL method
Purity
≥95% by SDS Page
≥95% monomer by analytical SEC
Formulation
This biosimilar antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
State of Matter
Liquid
Product Preparation
Recombinant biosimilar antibodies are manufactured in an animal free facility using only in vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Pathogen Testing
To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s recombinant biosimilar antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles.
Regulatory Status
Research Use Only (RUO). Non-Therapeutic.
Country of Origin
USA
Shipping
2-8°C Wet Ice
Additional Applications Reported In Literature ?
FA
N
IP
WB
ELISA
IF
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Description

Specificity
This non-therapeutic biosimilar antibody uses the same variable region sequence as the therapeutic antibody Tabalumab. Tabalumab neutralizes soluble human, cynomolgus monkey, and rabbit BAFF. Additionally, Tabalumab neutralizes membrane-bound BAFF. This product is for research use only.
Background
Tabalumab is a human monoclonal anti-B-cell activating factor (BAFF) antibody intended for the treatment of autoimmune diseases and B cell malignancies.1 BAFF is a membrane-bound, type II transmembrane protein that belongs to the tumor necrosis factor (TNF) ligand family and is the ligand for BR3, TACI, and BCMA. BAFF is an immunostimulant necessary for maintaining normal immunity. This cytokine has also been shown to play an important role in the proliferation and differentiation of B cells. An inadequate level of BAFF leads to immunodeficiency whilst an elevated level of BAFF causes unusually high antibody production that results in the development of autoimmune diseases such as systemic lupus erythematosus and rheumatoid arthritis. Additionally, BAFF has been found in renal transplant biopsies with acute rejection.2 Furthermore, BAFF may be a mediator of food-related inflammation, and is associated with multiple dietary ailments including celiac disease, insulin resistance, diabetes, and obesity.3 Interestingly, it is suspected that BAFF may be involved in non-IgE-mediated reactions because there is no known correlation between BAFF and IgE.4 More research is needed to unlock the enormous therapeutic potential for BAFF antagonists. This cost-effective, research-grade Anti-Human CD257 (BAFF) (Tabalumab) utilizes the same variable regions from the therapeutic antibody Tabalumab making it ideal for research projects.
Antigen Distribution
BAFF is expressed on various cell types including monocytes, dendritic cells and bone marrow stromal cells.
Ligand/Receptor
TACI, BCMA,APRIL ligand, BAFFR/BR3
PubMed
NCBI Gene Bank ID
UniProt.org
Research Area
Biosimilars
.
Cancer
.
Cell Biology
.
Costimulatory Molecules
.
Immuno-Oncology
.
Immunology
.
Signal Transduction
.
Stem Cell

Leinco Antibody Advisor

Powered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments.

Research-grade tabalumab biosimilars are not directly addressed in the provided search results, but the principles of using biosimilars as calibration standards or reference controls in pharmacokinetic (PK) bridging ELISAs can be inferred from general practices. Here's how biosimilars might be used in such assays:

Role of Biosimilars as Calibration Standards

Biosimilars can serve as calibration standards in PK assays due to their high degree of similarity to the reference biologic drug. This similarity ensures that the biosimilar can accurately reflect the pharmacokinetic behavior of the reference drug.

Use in Bridging ELISAs

  1. Calibration Curve Establishment: Biosimilars can be used to establish a calibration curve for measuring drug concentrations. This involves preparing standards of known concentrations of the biosimilar, which are then used to create a dose-response curve.

  2. Reference Controls: Biosimilars can also be used as reference controls to validate the assay performance. This ensures that the assay is precise, accurate, and reliable across different runs.

  3. Bioanalytical Equivalence: To ensure that the biosimilar is suitable for use as a calibration standard, it must demonstrate bioanalytical equivalence to the reference drug. This involves comparing the performance of the biosimilar to the reference product in the assay to confirm that both yield equivalent results.

Implementation in PK Bridging ELISAs

  • Single PK Assay Approach: Ideally, a single PK assay using a single analytical standard is preferred for measuring both biosimilar and reference products. This approach minimizes variability and avoids the need for complex crossover analyses.

  • Validation Studies: Validation studies are crucial to ensure that the assay is robust and can accurately quantify both the biosimilar and reference products. These studies involve assessing precision, accuracy, and robustness across multiple assays and analysts.

  • Bridging Format ELISA: A bridging format ELISA is often used to measure the presence of anti-drug antibodies or to quantify drug concentrations. This format involves using two distinct antibodies to capture and detect the drug, ensuring specificity and sensitivity.

In summary, while the specific use of tabalumab biosimilars as calibration standards or reference controls in PK bridging ELISAs is not detailed in the search results, the general principles of using biosimilars in such assays involve establishing bioanalytical equivalence, creating calibration curves, and ensuring assay performance through robust validation studies.

The primary models used to study tumor growth inhibition and characterize tumor-infiltrating lymphocytes (TILs) in the context of BAFF inhibition are not explicitly mentioned in the search results. However, based on the information provided, here's how these models could be applied:

Syngeneic Models

Syngeneic Tumor Models are widely employed in preclinical immunotherapy studies due to their immune-competent status and fully functional immune systems. These models are excellent for evaluating the efficacy of immunotherapeutic agents by analyzing tumor-immune interactions and TILs. Although the search results do not specifically mention the use of anti-BAFF antibodies in syngeneic models for tumor growth inhibition, such models are theoretically suitable for this purpose. They could be used to study how anti-BAFF antibodies modulate the immune response and affect TIL composition and function in the context of tumor growth.

Humanized Models

Humanized Models are used to mimic human immune responses and are particularly valuable for studying aspects of human immunology that are not easily replicable in animal models. These models typically involve the transplantation of human immune cells into immunocompromised mice, allowing researchers to study human immune responses in a controlled environment. While not specifically mentioned for anti-BAFF antibody studies, humanized models could potentially be used to explore the effects of BAFF inhibition on human TILs and tumor growth, providing insights into how these mechanisms might translate to human immunotherapy.

In summary, while the search results do not provide specific examples of using research-grade anti-BAFF antibodies in these models, both syngeneic and humanized models could be utilized to study the effects of BAFF inhibition on tumor growth and TILs. Syngeneic models are particularly useful for studying immune responses within a fully functional mouse immune system, while humanized models offer insights into human immune responses.

For a direct study on tumor growth inhibition and characterization of TILs using anti-BAFF antibodies, specific research would need to be conducted using these models.

While the search results do not provide specific information on the use of Tabalumab in conjunction with checkpoint inhibitors like anti-CTLA-4 or anti-LAG-3 biosimilars in immune-oncology models, it is possible to infer a general approach based on the mechanisms of these drugs and how researchers might combine them.

Understanding the Components

  1. Tabalumab: This is a monoclonal antibody targeting B-cell activating factor (BAFF), which plays a role in B-cell survival and homeostasis. It is used primarily in autoimmune diseases rather than cancer, but its mechanism could be relevant in modulating immune responses in cancer models.

  2. Checkpoint Inhibitors:

    • Anti-CTLA-4: Inhibits CTLA-4 receptors on T-cells, promoting T-cell activation and proliferation by preventing CTLA-4 from inhibiting T-cell activation.
    • Anti-LAG-3: Inhibits LAG-3 receptors, which are involved in the regulation and exhaustion of T-cells. Blocking LAG-3 can enhance T-cell responses.

Potential Synergistic Effects

Combining Tabalumab with checkpoint inhibitors could theoretically modulate immune responses in several ways:

  • Enhancing T-Cell Activity: By inhibiting CTLA-4 or LAG-3, checkpoint inhibitors enhance T-cell activation. If Tabalumab can modulate B-cell function, it might also influence T-cell responses indirectly, potentially enhancing the overall immune response against tumors.

  • Modulating Immune Microenvironment: BAFF, targeted by Tabalumab, could influence the tumor microenvironment by affecting B-cell populations, which might interact with T-cells. Combining with checkpoint inhibitors could further modulate this environment, enhancing antitumor effects.

  • Complex Immune-Onco Models: In complex models involving both B-cell and T-cell interactions, the combination could provide insights into how different immune components interact and how they might be targeted synergistically to improve cancer treatment outcomes.

However, as of the latest research, there is no specific evidence of using Tabalumab directly in conjunction with these checkpoint inhibitors for synergistic effects in immune-oncology models. Future studies would be needed to explore these potential synergies.

Example Experimental Design

  1. Model Setup: Use a cancer model where both B-cell and T-cell interactions play a significant role.

  2. Treatment Groups:

    • Control Group
    • Tabalumab alone
    • Checkpoint inhibitor alone (e.g., anti-CTLA-4 or anti-LAG-3)
    • Combination of Tabalumab and checkpoint inhibitor
  3. Outcome Measures:

    • Tumor growth
    • Immune cell infiltration (e.g., CD4, CD8 T-cells, B-cells)
    • B-cell and T-cell activation markers
    • Survival and quality of life metrics
  4. Analysis: Compare outcomes across treatment groups to identify synergistic effects and potential mechanisms of action.

This approach would allow researchers to evaluate whether combining Tabalumab with checkpoint inhibitors enhances antitumor immunity beyond what is achieved with either treatment alone.

In the context of immunogenicity testing, a Tabalumab biosimilar can be used in a bridging ELISA to monitor a patient's immune response by serving as either a capture or detection reagent. However, Tabalumab itself is not explicitly mentioned in the search results as being used in such a manner. Nonetheless, the general principles of using biosimilars in bridging ELISA assays can be applied:

Overview of Bridging ELISA

Bridging ELISA is a technique used to detect and quantify antibodies against therapeutic drugs, such as biologics and monoclonal antibodies. It involves using the drug itself or a biosimilar as both capture and detection reagents to identify anti-drug antibodies (ADAs) in patient samples.

Using Biosimilars in Bridging ELISA:

  1. Capture Reagent:

    • The biosimilar can be biotinylated and used to coat streptavidin-coated plates. This allows the capture of ADAs from patient serum.
    • The biotinylated biosimilar acts as a bait to bind ADAs, which will specifically bind to the therapeutic drug.
  2. Detection Reagent:

    • After capturing the ADAs, an enzyme-conjugated (e.g., HRP) version of the biosimilar is added. This enzyme-conjugated reagent binds to the captured ADAs, forming a "bridge" between the capture and detection steps.
    • The addition of a chromogenic substrate (e.g., TMB) allows for the quantification of the bound ADAs based on colorimetric changes.

Benefits and Considerations:

  • Sensitivity and Throughput: Bridging ELISA offers high sensitivity and can be optimized for high-throughput screening, making it suitable for large-scale clinical studies.
  • Specificity Challenges: The assay's specificity can be affected by matrix components in complex samples like human serum, necessitating the use of high-quality reagents and assay conditions.

Applicability to Tabalumab:

While Tabalumab is not specifically mentioned in the context of bridging ELISA in the search results, the principles outlined above can be applied if a Tabalumab biosimilar were used in such assays. The key steps involve biotinylation for capture and enzyme conjugation for detection, with the biosimilar serving as both to identify ADAs against Tabalumab.

To apply this method to Tabalumab, one would:

  • Biotinylate the Tabalumab biosimilar for use as the capture reagent.
  • Use an enzyme-conjugated version of the Tabalumab biosimilar as the detection reagent.
  • Follow the standard bridging ELISA protocol for ADA detection.

Conclusion

In summary, while specific details about using a Tabalumab biosimilar in a bridging ELISA are not provided in the search results, the general approach involves using biosimilars as capture and detection reagents to monitor patient immune responses against therapeutic drugs. This method is widely applicable to various biologics and can be adapted to any biosimilar, including those for Tabalumab, by following the standard bridging ELISA protocol.

References & Citations

1. Manetta, J. et al. (2014) J Inflamm Res. 7: 121–131
2. Clatworthy, MR. et al. (2013) Transplantation. 96(4): 413–420.
3. Lied, GA. and Berstad, A. (2011) Scand J Immunol. 73(1):1-7.
4. Büchler, JR. and Cano, MN. (1986) Jpn Heart J. 27(1):117-22.
Indirect Elisa Protocol
FA
Flow Cytometry
IF
Immunoprecipitation Protocol
N
General Western Blot Protocol

Certificate of Analysis

Formats Available

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Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.