Anti-Human CD5 (Clone UCHT-2) – Purified (PhenoCyler-Fusion (CODEX)® Ready)

Anti-Human CD5 (Clone UCHT-2) – Purified (PhenoCyler-Fusion (CODEX)® Ready)

Product No.: C505

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Clone
UCHT-2
Target
CD5
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Leu-1, Ly-1, T1, Tp67
Isotype
IgG1
Applications
IHC FF
,
PhenoCycler®

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Product Size
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CODEX® Details

Validation Notes
Clone UCHT-2 has been validated for use on CODEX® using human tonsil fresh frozen (FF) tissue.
Tissue Screened
Tonsil
Tissue Preparation
FF (Fresh Frozen)

Antibody and Reporter Details

Reactivity Species
Human
Host Species
Mouse
Concentration
0.5 mg/ml
Formulation
This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4.
Storage and Handling
This antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Applications and Recommended Usage?
Quality Tested by Leinco
CODEX® This CD5 (Clone UCHT-2) antibody is formulated to simplify the antibody preparation needed when performing a CODEX® barcode conjugate. The suggested concentration is 0.5 mg/ml.
RRID
AB_2893566
Country of Origin
USA
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Mouse Anti-Human CD5 (Clone UCHT-2) recognizes Human CD5. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Anti-Human CD5 recognizes a human T-lymphocyte surface membrane antigen (Mr 67 kDa).
Antigen Distribution
The CD5 antigen is present on approximately 70% of normal peripheral blood lymphocytes and >90% of all T-lymphocytes. T-lymphocytes in the thymus stain weakly while peripheral T-cells stain strongly. Monocytes, granulocytes and NK cells are not stained.1 Anti-CD5 also reacts with a distinct subset of normal B-lymphocytes, occasional cells in B-lymphocyte areas of spleen and lymph node, and most Ig+ B-chronic lymphoblastic leukemia (CLL) cells. The CD5 antigen has also been shown to be present on some lymphomas.
Background
Anti-Human CD5 can be used in research to study B-lymphocyte subsets or characterization of leukemias and lymphomas.

Antigen Details

Protein
Ligand/Receptor
CD72
PubMed
NCBI Gene Bank ID

References & Citations

1. Leukocyte Typing VI (1995) Oxford University Press 2. Leukocyte Typing III (1987) Oxford University Press 3. Beverly, P. C. L. et al. (1981) "Protides of the Biological Fluids," Vol. XXIX, Peeters, H. (ed.), Pergamon Press, Oxford, 653
IHC
PhenoCycler®
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Prod No.
Description
C2059
Products are for research use only. Not for use in diagnostic or therapeutic procedures.