Anti-Human CD5 (Clone UCHT-2) – PE

Pricing & Details

Product No.C116
Clone
UCHT-2
Protein
CD5
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Leu-1, Ly-1, T1, Tp67
Isotype
Mouse
IgG1
Applications
FA
,
FC
Prod No.
Size
Price
Avail.
Qty
Add to cart
C116-50 µg
50 µg
$99.00
In stock
Max:
Min: 1
Step: 1
C116-100 µg
100 µg
$176.00
In stock
Max:
Min: 1
Step: 1
C116-200 µg
200 µg
$302.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Data

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Mouse
Product Concentration
0.2 mg/ml
Formulation
This R-phycoerythrin (R-PE) conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This R-PE conjugate is stable when stored at 2-8°C. Do not freeze.
Country of Origin
USA
Shipping
Next Day 2-8°C
Excitation Laser
Blue Laser (488 nm) and/or Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this UCHT-2 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 106 cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application.
Additional Reported Applications For Relevant Conjugates ?
IHC (Frozen)
WB
For specific conjugates of this clone, review literature for suggested application details.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Mouse Anti-Human CD5 (Clone UCHT-2) recognizes Human CD5. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Anti-Human CD5 recognizes a human T-lymphocyte antigen (Mr 67 kDa).
Antigen Distribution
The CD5 antigen is present on approximately 70% of normal peripheral blood lymphocytes and >90% of all T-lymphocytes. T-lymphocytes in the thymus stain weakly while peripheral T-cells stain strongly. Monocytes, granulocytes and NK cells are not stained.1 Anti-CD5 also reacts with a distinct subset of normal B-lymphocytes, occasional cells in B-lymphocyte areas of spleen and lymph node, and most Ig+ B-chronic lymphoblastic leukemia (CLL) cells. The CD5 antigen has also been shown to be present on some lymphomas.
Background
Anti-Human CD5 can be used in research to study B-lymphocyte subsets or characterization of leukemias and lymphomas.

Antigen Details

Protein
Ligand/Receptor
CD72
PubMed
NCBI Gene Bank ID
Research Area
Costimulatory Molecules
.
Immunology

References & Citations

1. Leukocyte Typing VI (1995) Oxford University Press 2. Leukocyte Typing III (1987) Oxford University Press 3. Beverly, P. C. L. et al. (1981) "Protides of the Biological Fluids," Vol. XXIX, Peeters, H. (ed.), Pergamon Press, Oxford, 653 Phycobilliproteins are protected under the following patents: (U.S. patents No. 4,520,110 and 4,542,104, European patent no. 76695, Canadian patent No. 1,179,942 and Australian patent No. 548,440).
Flow Cytometry
Prod No.
Description
C2059

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.