Anti-Human CD5 [Clone UCHT-2] — Purified in vivo Functional GOLD™ Functional Grade
Anti-Human CD5 [Clone UCHT-2] — Purified in vivo Functional GOLD™ Functional Grade
Product No.: C1025
Clone UCHT-2 Target CD5 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Leu-1, Ly-1, T1, Tp67 Isotype Mouse IgG1 Applications FA , FC , IHC FF , in vivo , PhenoCycler® |
Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Recommended Isotype Controls Recommended Dilution Buffer Product Concentration ≥ 5.0 mg/ml Endotoxin Level < 1.0 EU/mg as determined by the LAL method Purity ≥95% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2893537 Applications and Recommended Usage? Quality Tested by Leinco FC The suggested concentration for this UCHT-2 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 106 cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application. Additional Reported Applications For Relevant Conjugates ? IHC (Frozen) WB For specific conjugates of this clone, review literature for suggested application details. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Mouse Anti-Human CD5 (Clone UCHT-2) recognizes Human CD5. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Anti-Human CD5 recognizes a human T-lymphocyte surface membrane antigen (Mr 67 kDa). Background Anti-Human CD5 can be used in research to study B-lymphocyte subsets or characterization of leukemias and lymphomas. Antigen Distribution The CD5 antigen is present on approximately 70% of normal peripheral blood lymphocytes and >90% of all T-lymphocytes. T-lymphocytes in the thymus stain weakly while peripheral T-cells stain strongly. Monocytes, granulocytes and NK cells are not stained.1 Anti-CD5 also reacts with a distinct subset of normal B-lymphocytes, occasional cells in B-lymphocyte areas of spleen and lymph node, and most Ig+ B-chronic lymphoblastic leukemia (CLL) cells. The CD5 antigen has also been shown to be present on some lymphomas. Ligand/Receptor CD72 PubMed NCBI Gene Bank ID UniProt.org Research Area Costimulatory Molecules . Immunology Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. Clone UCHT-2 is most commonly used in vivo in humanized mouse models to selectively target human CD5-positive cells, especially for immune cell depletion and immunological studies. Key in vivo applications in mice include:
These applications rely on humanized mice, which are engineered to express human immune cells and are required because clone UCHT-2 is highly specific for human CD5 and does not cross-react robustly with murine CD5. Standard experimental protocols involve antibody administration followed by assessment of human CD5+ cell depletion or immune modulation. Summary: In the literature, UCHT-2, an anti-CD5 antibody, is commonly used in conjunction with several other antibodies or proteins to identify and study various cell types, particularly T and B cells. Here are some of the other commonly used antibodies or proteins used with UCHT-2:
These combinations help in understanding the distribution and function of different lymphocyte subsets in health and disease. Key Scientific Findings from Clone UCHT-2Clone UCHT-2 is a murine monoclonal antibody broadly used in immunology research to specifically target and study the human CD5 antigen, a 67 kDa single-chain, type 1 transmembrane glycoprotein. Below are the main scientific findings and applications associated with this clone, as documented in the literature and product specifications. Specificity and Antigen Expression
Applications in Research
Technical and Clinical Relevance
Summary Table: Key Properties of UCHT-2 Clone
ConclusionClone UCHT-2 is a cornerstone reagent for immunophenotyping, enabling precise identification of CD5+ cell populations in both health and disease. Its specificity, validated across decades of use, supports its continued role in basic, applied, and translational immunology research. Dosing Regimens of Clone UCHT-2 in Mouse ModelsClone UCHT-2 is a widely used anti-human CD5 monoclonal antibody with applications in both in vitro assays (e.g., flow cytometry, immunohistochemistry) and in vivo research, such as humanized mouse models for immunology and oncology. However, detailed, standardized dosing regimens for clone UCHT-2 in various mouse models are not explicitly described in the available literature. Here is a synthesis of current knowledge and practical considerations: No Universal Dosing Guideline
Practical Considerations for Humanized Mouse Models
General Recommendations
Summary Table: Key Points
ConclusionDosing regimens for clone UCHT-2 in mouse models are not standardized and must be tailored to the specific experimental context. Researchers should base initial doses on those used for similar anti-human antibodies, then empirically optimize for their particular mouse model and research objectives. Collaboration with suppliers and reference to analogous studies can provide practical starting points, but rigorous pilot testing remains essential. References & Citations1. Leukocyte Typing VI (1995) Oxford University Press
2. Leukocyte Typing III (1987) Oxford University Press
3. Beverly, P. C. L. et al. (1981) "Protides of the Biological Fluids," Vol. XXIX, Peeters, H. (ed.), Pergamon Press, Oxford, 653 Technical ProtocolsCertificate of Analysis |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
