Anti-Human cMyc (Clone 9E10) – HRP

C-terminal region of human c-myc

This HRP-conjugated antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4, 1% BSA. <b> (Warning: Use of sodium azide as a preservative will inhibit the enzyme activity of horseradish peroxidase)</b>

Liquid

This horseradish peroxidase conjugated monoclonal antibody is stable when stored at 2-8°C. Do not freeze.

Research Use Only

2 – 8° C Wet Ice

IHC,
IP,
IF,
WB,
ELISA,
FC

9E10 activity is directed against human c-Myc and can cross-react with denatured murine c-Myc in some circumstances.

c-Myc is an essential transcription factor that belongs to the superfamily of basic helix-loop- helix DNA-binding proteins that function in normal embryogenesis, acquisition and maintenance of stem cell properties, metabolism, cellular division, and cell death^{1, 2}. c-Myc is also a proto-oncogene, originally identified in Burkitt lymphoma¹. Its dysregulation, by mutation or epigenetic modification, has been observed in over 50% of human cancers³. c-Myc regulates various cancer cellular functions, including cell cycle, survival, proliferation, and metabolic reprogramming¹, and is associated with tumor aggression and chemoresistance³. c-Myc has been suggested as a target for cancer immunotherapy ²and various methods of inhibition have been studied^{1, 2, 3}.

9E10 was generated by immunizing a BALB/c mouse with a synthetic peptide corresponding to residues 408-432 of human c-Myc conjugated to keyhole limpet hemocyanin via the cysteine residues⁴. Splenocytes were fused with SP2 myeloma cells. 9E10 failed to immunoprecipitate protein from Colo 320 HSR cell extracts during screening but did detect c-Myc in Western blotting. The 9E10 epitope has become a well-known affinity tag used in recombinant protein expression, i.e., the myc-tag⁵. The target epitope is the C terminal EQKLISEEDL peptide with the core sequence being LISE⁵and the crystal structure and binding mode have been solved⁶. 9E10 detection has highly variable epitope recognition that is dependent on neighboring sequence context⁷. Additionally, cross-reactivity of 9E10 has been observed in murine cell lines in a fluorescence assay and by Western blotting⁸.

c-Myc is ubiquitously expressed during tissue development and in a wide variety of tumors. c-Myc is often studied in embryonic stem cells and induced pluripotent stem cells. c-Myc is mainly associated with cell nuclei.

P09416

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