Antibody DetailsProduct DetailsReactivity Species Human Host Species Goat Immunogen NS0-Derived Recombinant Human DNAM-1 Extracellular Domain Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present. Storage and Handling The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2829938 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Human DNAM-1 this polyclonal antibody can be used at a concentration of 0.1 - 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Human DNAM-1 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Other Applications Reported In Literature ? Neutralization: This antibody is useful for neutralization of Human DNAM-1 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human DNAM-1 (at 20 ng/ml) is 0.5 - 1.5 µg/ml. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Goat Anti-Human DNAX Accessory Molecule 1 (DNAM-1) recognizes Human DNAM-1. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background DNAX accessory molecule 1 (DNAM-1), is a tyrosine-phosphorylated signal-transducing molecule belonging to the immunoglobulin superfamily and participates in primary adhesion during cytotoxic T lymphocyte-mediated cytotoxicity. <sup<1 DNAM-1 is a functional glycoprotein expressed on immune cells, such as natural killer and T-cells. It is activated upon interaction with its biological ligands, the poliovirus receptor and nectin-2. Signaling cascades involving stimulation of DNAM-1 lead to various biological responses, including target cell lysis and immune cell activation. DNAM-1 appears to be an integral molecule in immune responses in cancer, allergic inflammatory disorders and autoimmune diseases.2 Antigen DetailsProtein PubMed NCBI Gene Bank ID UniProt.org References & Citations1. Phillips, JH. et al.(1996) Immunity. 4(6):573-81. 2. Levi-Schaffer, F. et al. (2008) Curr Opin Investig Drugs. 9(5):491-6. Technical Protocols |
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