Anti-Human E-Cadherin Antibody (42027)

Anti-Human E-Cadherin Antibody (42027)

Product No.: 42027

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Clone
SHE78-7
Target
E-Cadherin
Formats AvailableView All
Product Type
Monoclonal
Alternate Names
CAM 120/80, Epithelial cadherin, E-cadherin, Uvomorulin, CD antigen CD324 [Cleaved into: E-Cad/CTF1; E-Cad/CTF2; E-Cad/CTF3]
Isotype
IgG2a
Applications
ELISA
,
FACS
,
IF
,
IHC
,
WB

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Antibody Details

Product Details

Reactivity Species
Human
Host Species
Mouse
Immunogen
Human placenta E-Cadherin
Formulation
PBS, pH 7.4, lyophilized.
State of Matter
Lyophilized
Product Preparation
Purified by immunoaffinity chromatography
Storage and Handling
Lyophilized antibody is stable at 4°C for 2 years. Store the reconstituted solution in aliquots at -20°C for one year or at 4°C for 6 months after addition of 0.1% sodium azide by the user. Avoid repeated freeze-thaw cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
ELISA: 2ug/ml

Western Blotting

Flow : 2ug/ml, reducing and non-reducing conditions

Flow Cytometry: 1ug/ml.

Immunohistochemistry: 2ug/ml, paraffin- embedded and frozen tissue sections.

Immunofluorescence: 1ug/ml.Cell-Cell Contact Inhibition Assays: 1ug/ml.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Mouse Monoclonal Antibody specific to Human E-cadherin and does not react with rat E-Cadherin. This antibody inhibits E-Cadherin dependent cell-cell contact.

Antigen Details

Function
Cadherins are calcium-dependent cell adhesion proteins (PubMed:11976333). They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells (PubMed:11976333). Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7. {PubMed:11976333, PubMed:16417575}.; E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production. {PubMed:16417575}.; (Microbial infection) Serves as a receptor for Listeria monocytogenes; internalin A (InlA) binds to this protein and promotes uptake of the bacteria. {PubMed:10406800, PubMed:17540170, PubMed:8601315}.
NCBI Gene Bank ID
UniProt.org
Research Area
Cell Adhesion

References & Citations

Indirect Elisa Protocol
FACS
IF
IHC
General Western Blot Protocol

Formats Available

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Prod No.
Description
42027
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.