Anti-Human Eotaxin-3 (Clone 115002)
Anti-Human Eotaxin-3 (Clone 115002)
Product No.: E140
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Clone 115002 Target Eotaxin-3 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names SCYA26, MIP-4 Alpha, Eotaxin-3, IMAC, MIP-4a, TSC-1, CCL26 Isotype IgG1 Applications ELISA Cap , N , WB |
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Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Immunogen E. coli-Derived Recombinant Human CCL26 (Accession # Q9Y258) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2829990 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Human Eotaxin-3 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for rhCCL26 is approximately 0.5 ng/lane and 25 ng/lane under non-reducing and reducing conditions, respectively. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. ELISA Capture: Suitable for use at concentration of 2-8 µg/mL. Use in combination with: Detection Reagent: Human CCL26/Eotaxin‑3 Biotinylated Antibody. Standard: Recombinant Human CCL26/Eotaxin-3 Protein. Additional Applications Reported In Literature ? Neutralization: This antibody is useful for neutralization of Human Eotaxin-3 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human Eotaxin-3 (at 1 ng/ml) is 2-8 µg/ml. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Mouse Anti-Human Eotaxin-3 recognizes Human Eotaxin-3. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Background Eotaxin-3/CCL-26 belongs to a family of CC chemokines, which are potent chemoattractants for eosinophils that signal through the CCR3 receptor. 1 It is produced by endothelial cells stimulated with IL-4 or IL-13. Eotaxin-3 selectively targets cells expressing CCR3, including eosinophils, basophils, T cells and monocytes. It has been reported that eotaxin-3 is an antagonist for CCR2.2 Eotaxin-3 has similar activity to Eotaxin and Eotaxin-2, but the three Eotaxins share only a low degree of sequence homology. Eotaxin-3 is up-regulated in the context of a T helper 2 mediated inflammatory bowel disease via the signal transducer and activator of transcription 6, thus suggesting that the intestinal epithelium actively participates in the recruitment of eosinophils at the site of inflammation.1 Antigen DetailsPubMed References & Citations1. Cuber, JC.et al. (2005) Int J Biochem Cell biol. 37: 2559 2. Gerber, B. et al. (2004) J Biol Chem. 279: 23357 Technical Protocols N  |
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Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
