E. coli-Derived Recombinant Human CCL11
<0.1 EU/µg as determined by the LAL method
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Applications and Recommended Usage ?
(Quality Tested by Leinco)
IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 8.0 µg/ml. The recommended secondary antibody for IHC is PN:M114. For chromogenic detection with high signal and low background use PN:D100 or PN:K107.
Neutralization: This antibody is useful for neutralization of Human CCL11 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human CCL11 (at 10 ng/ml) is 1-5 µg/ml.
Western Blotting: To detect Human CCL11 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human CCL11 is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Mouse Anti-Human Eotaxin (Clone 43911) recognizes an epitope on Human Eotaxin. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
The CC-chemokine eotaxin is a potent eosinophil chemoattractant that stimulates recruitment of eosinophils from the blood to sites of allergic inflammation.1 Eotaxin and related molecules are potentially important endogenous signaling substances in allergic reactions.2 Eotaxin plays an important role not only by attracting eosinophils to the site of inflammation but also by damaging tissue by its capacity to induce the release of reactive oxygen species.3
References & Citations
1. Rankin SM. et al. (1998) Blood. 91:2240 2. Williams TJ. et al. (1993) Biochem Biophys Res Commun. 197:1167 3. Kapp A. et al. (1996) Eur J Immunol. 26:1919
Products are for research use only. Not for use in diagnostic or therapeutic procedures.