Anti-Human Eotaxin

Product No.: C1397


Clone 43911 Target Eotaxin Formats AvailableView All Product Type Monoclonal Antibody Alternate Names CCL11, MGC22554, SCYA11, Eosinophil Chemotactic Protein Isotype IgG1 Applications ELISA Cap , IHC FFPE , N , WB


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Antibody Details Product Details Reactive Species Human Host Species Mouse Immunogen E. coli-Derived Recombinant Human CCL11 Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2828454 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Human CCL11 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human CCL11 is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. ELISA Capture: Suitable for use at concentration of 2-8 µg/mL. Use in combination with: Detection Reagent: Human CCL11/Eotaxin Biotinylated Antibody. Standard: Recombinant Human CCL11/Eotaxin Protein. Additional Applications Reported In Literature ? IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 8.0 µg/ml. The recommended secondary antibody for IHC is PN:M114. For chromogenic detection with high signal and low background use PN:D100 or PN:K107. Neutralization: This antibody is useful for neutralization of Human CCL11 bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Human CCL11 (at 10 ng/ml) is 1-5 µg/ml. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Description Specificity Mouse Anti-Human Eotaxin (Clone 43911) recognizes an epitope on Human Eotaxin. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Background The CC-chemokine eotaxin is a potent eosinophil chemoattractant that stimulates recruitment of eosinophils from the blood to sites of allergic inflammation.¹ Eotaxin and related molecules are potentially important endogenous signaling substances in allergic reactions.² Eotaxin plays an important role not only by attracting eosinophils to the site of inflammation but also by damaging tissue by its capacity to induce the release of reactive oxygen species.³ PubMed Eotaxin References & Citations 1. Rankin SM. et al. (1998) Blood. 91:2240 2. Williams TJ. et al. (1993) Biochem Biophys Res Commun. 197:1167 3. Kapp A. et al. (1996) Eur J Immunol. 26:1919 View product citations for antibody C1397 on CiteAb Technical Protocols IHC FFPE N Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Human

Host Species

Mouse

Immunogen

E. coli-Derived Recombinant Human CCL11

Endotoxin Level

<0.1 EU/µg as determined by the LAL method

Formulation

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

Storage and Handling

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2828454

Applications and Recommended Usage?
Quality Tested by Leinco

Western Blotting: To detect Human CCL11 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human CCL11 is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
ELISA Capture: Suitable for use at concentration of 2-8 µg/mL. Use in combination with: Detection Reagent: Human CCL11/Eotaxin Biotinylated Antibody. Standard: Recombinant Human CCL11/Eotaxin Protein.

Additional Applications Reported In Literature ?

IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 8.0 µg/ml. The recommended secondary antibody for IHC is PN:M114. For chromogenic detection with high signal and low background use PN:D100 or PN:K107.
Neutralization: This antibody is useful for neutralization of Human CCL11 bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Human CCL11 (at 10 ng/ml) is 1-5 µg/ml.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Mouse Anti-Human Eotaxin (Clone 43911) recognizes an epitope on Human Eotaxin. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Background

The CC-chemokine eotaxin is a potent eosinophil chemoattractant that stimulates recruitment of eosinophils from the blood to sites of allergic inflammation.¹
Eotaxin and related molecules are potentially important endogenous signaling substances in allergic reactions.² Eotaxin plays an important role not only by attracting eosinophils to the site of inflammation but also by damaging tissue by its capacity to induce the release of reactive oxygen species.³

PubMed

Eotaxin

References & Citations

1. Rankin SM. et al. (1998) Blood. 91:2240 2. Williams TJ. et al. (1993) Biochem Biophys Res Commun. 197:1167 3. Kapp A. et al. (1996) Eur J Immunol. 26:1919

View product citations for antibody C1397 on CiteAb

IHC FFPE

Certificate of Analysis

Request COA

Prod No.	Description
C1397	Anti-Human Eotaxin
E182	Anti-Human Eotaxin (CT)
E183	Eotaxin Blocking Peptide
E202	Recombinant Mouse Eotaxin
E306	Mouse Eotaxin ELISA Development Kit
E304	Human Eotaxin ELISA Development Kit

Formats Available

Prod No.	Description
C1397	Anti-Human Eotaxin

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

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Anti-Human Eotaxin