Anti-Human Ephrin-A4 (Clone 135305)

Purified Recombinant Human Ephrin-A4 (Accession # P52798)

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

Next Day Ambient

Immunohistochemistry: Suitable for use at concentration of 5-25 µg/mL.

Mouse Anti-Human Ephrin-A4 (Clone 135305) recognizes an epitope on Human Ephrin-A4. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Ephrin-A4 is a glycoinositol phospholipid membrane anchor containing ephrin found in variety of adult tissue such as kidney, lung, colon and ovary. Ephrin-A4 is a ligand for the erythropoietin-producing hepatocellular (Eph) receptor family of tyrosine kinases.¹ Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. Ephrin-A4 encodes an EFNA class ephrin. Ephrin-A4 (s) may play a role in the interaction between activated B lymphocytes and dendritic cells in human tonsils.

1. Logtenberg, T. et al. (2000) Blood 95: 221