Anti-Human FGF-19

Anti-Human FGF-19

Product No.: F1061

[product_table name="All Top" skus="F1061"]

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Product Type
Polyclonal Antibody
Alternate Names
Fibroblast Growth Factor-19, FGFJ

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Antibody Details

Product Details

Reactive Species
Host Species
E. coli-Derived Recombinant Human FGF-19
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
State of Matter
Storage and Handling
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Human FGF-19 this polyclonal antibody can be used at a concentration of 0.5 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Human FGF-19 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Additional Applications Reported In Literature ?
Blocking: Approximately 2-8 μg/ml of this antibody will block 50% of the binding of 200 ng/ml of rhFGF-19 to immobilized rhFGF R4/Fc chimera (100 μL of a 2 μg/ml solution was coated in each well) in a functional ELISA.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.


Goat Anti-Human Fibroblast Growth Factor 19 (FGF-19) recognizes Human FGF-19. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Fibroblast growth factor 19, also known as FGF19 is an atypical member of the fibroblast growth factor (FGF) family of proteins. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes including embryonic development cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF19 uniquely binds to FGF receptor 4 (FGFR4).1

The FGF19 subfamily has reduced heparin binding resulting from a disrupted beta-trefoil domain. Reduced heparin binding allows these FGFs to diffuse beyond their site of origin and act as endocrine hormones. This family of FGFs is regulated, at least in part, by nuclear hormone receptors.2 Inactivation of FGF19 could be beneficial for the treatment of colon cancer, liver cancer and other malignancies involving interaction of FGF19 and FGFR4.3

Antigen Details

NCBI Gene Bank ID

References & Citations

1. French, DM. et al. (2008) Cancel Res. 68: 5086
2. Jones, S. et al. (2008) Mol Pharm. 5: 42
3. French, DM. et al. (2008) Oncogene 27: 85
General Western Blot Protocol
Products are for research use only. Not for use in diagnostic or therapeutic procedures.