Anti‑Human glycoprotein hormone α‑subunit (pan‑hCG/FSH/LH) (Clone 176) — HRP

Purified Recombinant Human FSH (>98%)

This horseradish peroxidase conjugate is supplied in a specially formulated stabilization diluent.(Leinco Prod. No.: S509)

Stable for at least one year when stored at 2°C - 8°C. Do not freeze.

hCG Test Paired with Leinco Prod. No. C102, Clone No. 151: ihCG 100%, BhCG <1%, LH <4%, FSH <2%, TSH <1%. Paired with Leinco Prod. No. C103, Clone No. 152: ihCG 100%, BhCG <1%, LH <2%, FSH <1%, TSH <1%. Paired with Leinco Prod. No. C104, Clone No. 208: ihCg 100%, BhCG <1%, LH <1%, FSH <1%, TSH <1%. FSH Test Paired with Leinco Prod. No. F102, Clone No. 181: FSH 100%, BhCG <1%, LH <4%, ihCG **, TSH <1% ** 3,000 mIU/ml hCG causes 4.5% decrease in observed FSH. At higher concentrations of hCG, observed FSH reduced to zero. LH Test Paired with Leinco Prod. No. L100, Clone No. 196: LH 100%, BhCG <1%, FSH <1%, ihCG 57%, TSH <1%. Paired with Leinco Prod. No. L101, Clone No. 199: LH 100%, BhCG <1%, FSH <1%, ihCG 7%***, TSH <1%, *** 3,000 mIU/ml hCG causes 6.5% increases in observed LH.

2-8°C

Clone 176 was generated using recombinant human FSH as immunogen and recognizes the glycoprotein hormone α‑subunit, enabling detection of FSH as well as cross‑reactive binding to LH and hCG in immunoassay formats, consistent with a pan‑glycoprotein hormone α‑subunit specificity profile.

Follicle‑stimulating hormone (FSH), luteinizing hormone (LH), thyroid‑stimulating hormone (TSH), and human chorionic gonadotropin (hCG) are heterodimeric glycoprotein hormones composed of a common α‑subunit paired with a hormone‑specific β‑subunit. The shared α‑subunit carries conserved epitopes that allow pan‑reactive monoclonal antibodies to recognize multiple members of this hormone family, while the unique β‑subunits confer biological and receptor specificity to each hormone. Clone 176 was generated using recombinant human FSH as immunogen and recognizes the glycoprotein hormone α‑subunit, enabling detection of FSH as well as cross‑reactive binding to LH and hCG in immunoassay formats, consistent with a pan‑glycoprotein hormone α‑subunit specificity profile defined in in‑house testing.

1.) Ramabhadran TV, Reitz BA, Tiemeier DC. Synthesis and glycosylation of the common alpha subunit of human glycoprotein hormones in mouse cells. Proc Natl Acad Sci U S A. 1984 Nov;81(21):6701-5. doi: 10.1073/pnas.81.21.6701. PMID: 6208554; PMCID: PMC391998.