Anti-Human G-CSF – Biotin

Pricing & Details

Product No.G115
Protein
G-CSF
Formats AvailableView All
Product Type
Polyclonal Antibody
Alternate Names
Granulocyte Colony Stimulating Factor, CSF-3, MGI-1G, GM-CSF Beta, Pluripoietin
Applications
ELISA Det
,
WB
Prod No.
Size
Price
Avail.
Qty
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G115-50 µg
50 µg
$525.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Goat
Immunogen
Purified Recombinant Human G-CSF (>98%)
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.4 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
Storage and Handling
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Human G-CSF this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Human G-CSF is 5.0 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:G123.
ELISA Sandwich Assay: This antibody can be used as the detection antibody in a sandwich ELISA at a concentration of approximately 0.1-0.4 µg/ml when used in conjunction with PN:G664 as the capture antibody at 2-8 µg/ml and an optimal second step reagent such as PN:A106 for the detection of Human G-CSF.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rabbit Anti-Human Granulocyte Colony-Stimulating Factor (G-CSF) recognizes Human Granulocyte Colony-Stimulating Factor. This antigen affinity purified polyclonal antibody was purified using immobilized antigen agarose chromotography.
Background
Granulocyte colony-stimulating factor (G-CSF or GCSF), also known as G-CSF colony-stimulating factor 3 (CSF 3) is a colony-stimulating factor hormone. It is a glycoprotein, growth factor or cytokine produced by a number of different tissues to stimulate the bone marrow to produce granulocytes and stem cells. G-CSF then stimulates the bone marrow to release them into the blood. It also stimulates the survival, proliferation, differentiation, and function of neutrophil precursors and mature neutrophils. G-CSF is produced by endothelium, macrophages, and a number of other immune cells. It stimulates the production of white blood cells. G-CSF is also used to increase the number of hematopoietic stem cells in the blood of the donor before collection by leukapheresis for use in hematopoietic stem cell transplantation.

Antigen Details

Protein
PubMed
NCBI Gene Bank ID
Indirect Elisa Protocol
General Western Blot Protocol
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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