Anti-Mouse G-CSF (Clone 67604)

Pricing & Details

Product No.G669
Clone
67604
Protein
G-CSF
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
CSF-3, MGI-1G, GM-CSF Beta, Pluripoietin, Granulocyte Colony Stimulating Factor
Isotype
Rat
IgG1
Applications
ELISA Cap
,
FC
,
N
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
G669-25 µg
25 µg
$205.00
In stock
Max:
Min: 1
Step: 1
G669-100 µg
100 µg
$389.00
In stock
Max:
Min: 1
Step: 1
G669-500 µg
500 µg
$920.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Mouse
Host Species
Rat
Immunogen
Purified Recombinant Mouse G-CSF (>98%)
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is PN:G140 at a concentration of approximately 50 ng/ml. A suggested standard for this assay is PN:G129.
Western Blotting: To detect Mouse G-CSF this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The detection limit for Mouse G-CSF is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:G129.
Flow Cytometry: Suitable for use at concentration of 0.25 µg/106 cells.
Other Applications Reported In Literature ?
Neutralization: This antibody is useful for neutralization of Mouse G-CSF bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Mouse G-CSF (at 0.125 ng/ml) is 0.01 - 0.03 µg/ml.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rat Anti-Mouse Granulocyte Colony Stimulating Factor (G-CSF) recognizes an epitope on Mouse G-CSF. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Less than 0.06% cross-reactivity with rhG-CSF, rhCNTF, rmIL-6, rmLIF and rmOSM was observed.
Background
Granulocyte colony-stimulating factor (G-CSF or GCSF), also known as G-CSF colony-stimulating factor 3 (CSF 3) is a colony-stimulating factor hormone. It is a glycoprotein, growth factor or cytokine produced by a number of different tissues to stimulate the bone marrow to produce granulocytes and stem cells. G-CSF then stimulates the bone marrow to release them into the blood. It also stimulates the survival, proliferation, differentiation, and function of neutrophil precursors and mature neutrophils. G-CSF is produced by endothelium, macrophages, and a number of other immune cells. It stimulates the production of white blood cells. G-CSF is also used to increase the number of hematopoietic stem cells in the blood of the donor before collection by leukapheresis for use in hematopoietic stem cell transplantation.

Antigen Details

Protein
PubMed
Elisa Sandwich Protocol
Flow Cytometry
General Western Blot Protocol

Formats Available

Prod No.
Description
G669
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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