Anti-Human GFRα-2
Anti-Human GFRα-2
Product No.: G683
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Target GFR alpha-2 Product Type Polyclonal Antibody Alternate Names Glial Cell Line-Derived Neurotropic Factor soluble Receptor Alpha 2, GDNFRB, NRTNR-Alpha, NTNRA, RETL2, TRNR2 Applications B , ELISA Indirect , IHC FFPE , WB |
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Antibody DetailsProduct DetailsReactive Species Human Host Species Goat Immunogen NS0 - Derived Recombinant Human GFR α-2 Extracellular Domain Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830272 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Human GFR α-2 this polyclonal antibody can be used at a concentration of 0.1 - 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Human GFR α-2 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Additional Applications Reported In Literature ? IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 15 µg/ml. The recommended secondary antibody for IHC is PN:G505. For chromogenic detection with high signal and low background use PN:D100 or PN:K107. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Goat Anti-Human Glial Cell-Line derived Neurotrophic Factor soluble Receptor Alpha 2 (GFR α-2) recognizes Human GFR α-2. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background Glial cell line-derived neurotrophic factor family receptor alpha-2, also known as GFRA2, is the cognate co-receptor for the neurotrophic factor neurturin that is selectively expressed by isolectin B(4) (IB 4)-binding nociceptive sensory neurons.1 GFRA2 is required for the development of the myenteric nerve plexus and is an excellent candidate gene for Hirschsprung disease (HSCR).2 GFRalpha2 signaling is also needed for development of the parasympathetic islet innervation that is critical for vagally induced hormone secretion.3 Antigen DetailsPubMed NCBI Gene Bank ID UniProt.org References & Citations1. Lewin, GR. et al. (2002) J Physiol. 545: 43 2. Mulligan, LM. et al. (2001) Human Genetics 108: 409 3. Herzig, KH. et al. (2005) Diabetes 54: 1324 Technical ProtocolsB  IHC FFPE  |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
