Anti-Human Histone H3 Nonmethyl (Lys9) – HRP
Anti-Human Histone H3 Nonmethyl (Lys9) – HRP
Product No.: H458
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Clone 9B1-2G6 Target Histone H3 Nonmethyl (Lys9) Formats AvailableView All Product Type Hybridoma Monoclonal Antibody Alternate Names Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l Isotype Mouse IgG1 κ Applications WB , IHC-P |
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Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Immunogen Synthetic, branched, KLH-conjugated peptide corresponding to human unmethylated Histone H3 amino acids surrounding Lys9 Product Concentration 0.5 mg/ml Formulation This HRP-conjugated antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4, 1% BSA. (Warning: Use of sodium azide as a preservative will inhibit the enzyme activity of horseradish peroxidase) State of Matter Liquid Storage and Handling This horseradish peroxidase conjugated monoclonal antibody is stable when stored at 2-8°C. Do not freeze. Regulatory Status Research Use Only Country of Origin USA Shipping 2 – 8° C Wet Ice Additional Applications Reported In Literature ? DOT, IHC-P, WB Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity 9B1-2G6 activity is directed against unmethylated human histone H3 (Lys9) when
Ser10 is phosphorylated (H3K9me0). 9B1-2G6 also detects mono-/di-methyl-Histone H3 (Lys4),
Acetyl-Histone H3 (Lys14), H3K4me3, Histone H3 K14ac, Histone H3K4ac, and Histone H3
S10ph. Background Nucleosomes are the basic building blocks of chromatin1. Nucleosomes are composed of ~147
bp of DNA wrapped around a histone octamer. This octamer is composed of a central (H3-H4)
tetramer that is flanked by two H2A-H2B dimers. Histones H2B and H4 are relatively conserved,
but many variants of H2A and H3 exist. H3 variants in humans include H3.1, H3.2, H3.3,
H3t/H3.4, H3.5, H3.Y, H3.X, CENP-A, H3.6, H3.7 and H3.8. Both H3.1 and H3.2, which differ
by a single amino acid at residue 96, are expressed in S-phase during replication. In contrast,
H3.3 is expressed throughout interphase and is enriched over actively transcribed genes and
repetitive DNA elements. The other variants are less studied, but all histones are heavily
regulated at transcriptional and post-translational levels, including methylation, acetylation, and
phosphorylation. Histone modification status has a direct biophysical effect on gene expression
and chromatin structure. Lysines may be unmethylated (me0), monomethylated (me1),
demethylated (me2), and trimethylated (me3), each with its own regulatory effects. 9B1-2G6 was raised against a synthetic, branched, KLH-conjugated peptide corresponding to human unmethylated Histone H3 amino acids surrounding Lys9. 9B1-2G6 targets H3 when unmethylated (me0) at Lys9. This antibody is also known as the Histone H3K9me0 antibody. Antigen Distribution H3 is expressed in nucleosomes. Ligand/Receptor H2A, H2B, H4 NCBI Gene Bank ID UniProt.org Research Area Neuroscience . Signal Transduction . Chromatin . DNA . Epigenetics References & Citations1 Scott WA, Campos EI. Front Cell Dev Biol. 8:701. 2020. 2 Longarini EJ, Dauben H, Locatelli C, et al. Mol Cell. 83(10):1743-1760.e11. 2023. Technical Protocols IHC-P Certificate of Analysis |
Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
