Anti-Human HLA-DQ (MHC Class II) – FITC

Anti-Human HLA-DQ (MHC Class II) – FITC

Product No.: H137

[product_table name="All Top" skus="H137"]

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Clone
1a3
Target
HLA-DQ Monomorphic
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
HLA-DQ Monomorphic
Isotype
IgG2a

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Product Size
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Antibody Details

Product Details

Reactive Species
Human
Host Species
Mouse
Product Concentration
0.2 mg/ml
Formulation
This Fluorescein (FITC) conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This Fluorescein conjugate is stable when stored at 2-8°C. Do not freeze.
Country of Origin
USA
Shipping
Next Day Ambient
Excitation Laser
Blue Laser (490 nm)
Applications and Recommended Usage?
Quality Tested by Leinco
FC
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone 1a3 recognizes a monomorphic epitope on human HLA-DQ1. It does not cross-react with HLA-DR or HLA-DP.
Antigen Distribution
HLA-DQ is expressed on antigen-presenting cells, including macrophages, monocytes, DCs, and B cells, and activated T cells.
Background
HLA-DQ antibody, clone 1a3, recognizes the major histocompatibility complex (MHC) class II molecule Human Leukocyte Antigen - DQ isotype (HLA-DQ). MHC class II is constitutively expressed on human professional antigen-presenting cells (APCs), including macrophages/monocytes, dendritic cells (DCs), and B cells, and is induced on T cells upon activation2. HLA-DQ consists of two transmembrane proteins, a 35 kDa α (heavy) chain and 29 kDa β (light) chain3 encoded by the HLA-DQA1 and HLA-DQB1 genes, respectively, located in the HLA complex of chromosome 6. The N-terminal α1 and β1 domains form the antigen-binding groove, which binds 13-25 aa peptides derived from exogenous antigens4. On APCs, MHC class II plays a critical role in the adaptive immune response by presenting phagocytosed antigens to helper CD4 T cells. The T cell receptor (TCR)/CD3 complex of CD4 T cells interacts with peptide-MHC class II, which induces CD4 T cell activation leading to the coordination and regulation of other effector cells. CD4 molecules also bind to MHC class II, which helps augment TCR signaling5. It has also been demonstrated that MHC class II express on activated T cells are capable of antigen presentation6 and can transduce signals into T cells, enhancing T cell proliferation and activity7. Specific alleles of HLA-DQ are associated with autoimmune diseases, including celiac disease8 and type 1 diabetes9, and graft-versus-host disease10.

Antigen Details

Ligand/Receptor
CD3/TCR, CD8
NCBI Gene Bank ID
Research Area
Immunology
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Innate Immunity

References & Citations

1. Shookster L, et al. (1987) Hum Immunol. 20(1):59-70
2. Holling TM, Schooten E, van Den Elsen PJ. (2004) Hum Immunol. 65(4):282-90
3. Mitaksov V, Fremont DH. (2006) J Biol Chem. 281(15):10618-25
4. Wieczorek M, et al. (2017) Front Immunol. 8:292
5. Artyomov MN, et al. (2010) Proc Natl Acad Sci USA. 107(39):16916-16921
6. Barnaba V, et al (1994) Eur J Immunol. 24(1):71-5
7. Di Rosa F, et al. (1993) Hum Immunol. 38(4):251-60
8. Castaño L, et al. (2004) J Pediatr Gastroenterol Nutr. 39:80–84
9. Cucca F, et al. (1993) Hum Immunol. 37:85 –94
10. Petersdorf EW, (1996) Proc Natl Acad Sci USA. 93(26):15358-63

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.