Anti-Human IGFBP-rp1

Anti-Human IGFBP-rp1

Product No.: I-875

[product_table name="All Top" skus="I-875"]

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Clone
192520
Target
IGFBP-rp1
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Insulin-Like Growth Factor Binding Protein-Related Protein-1
Isotype
IgG1
Applications
ELISA
,
WB

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Data

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Antibody Details

Product Details

Reactive Species
Human
Host Species
Mouse
Immunogen
Purified Recombinant Human IGFBP-rp1 (Accession # AAA16187)
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
ELISA: This antibody can be used in an indirect detection ELISA at 0.5-1.0 µg/ml with a suitable second step reagent such as PN:M114. The detection sensitivity of this indirect ELISA for Human IGFBP-rp1 is approximately 2 ng/well.
Western Blotting: To detect Human IGFBP-rp1 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human IGFBP-rp1 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:I-335.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Mouse Anti-Human Insulin-like growth factor binding protein-related protein-1 (IGFBP-rp1) (Clone 192520) recognizes an epitope on Human IGFBP-rp1. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Antigen Details

PubMed
NCBI Gene Bank ID
Indirect Elisa Protocol
General Western Blot Protocol
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Formats Available

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Prod No.
Description
I-875
Products are for research use only. Not for use in diagnostic or therapeutic procedures.