Anti-Human IgM µ-Chain Specific (Clone HB57) – Purified in vivo GOLD™ Functional Grade
Anti-Human IgM µ-Chain Specific (Clone HB57) – Purified in vivo GOLD™ Functional Grade
Product No.: I-1202
Clone HB57 Target Human Immunoglobulin IgM µ-Chain Specific Formats AvailableView All Product Type Monoclonal Antibody Isotype Mouse IgG1 Applications ELISA , FC , in vivo |
Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Recommended Isotype Controls Recommended Dilution Buffer Product Concentration ≥ 5.0 mg/ml Endotoxin Level < 1.0 EU/mg as determined by the LAL method Purity ≥95% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Cross Reactivity Cross-reactivity by ELISA against human myeloma proteins: Human IgG1: 100% Human IgG2: 100% Human IgG3: 100% Human IgG4: 100% Human IgM: <0.2% Human IgA: <0.1% Human IgE: <0.1% There is no detectable binding to bovine, goat, horse sheep IgG. NOTE: This Anti-Human IgG, Fc Fragment Specific (Clone HB57) when conjugated to HRP is validated for use in Enzyme Immunoassay for the detection of Human IgG, µ-Chain Specific (Part No.: I-1201) Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2893816 Applications and Recommended Usage? Quality Tested by Leinco FC This antibody has been quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. ELISA This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 5 µg/ml (100 µl/well) µg/ml. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Anti-Human IgM (µ chain specific) monoclonal antibody reacts with human IgM through an epitope on the heavy chain. This antibody is non-reactive with IgG, IgA or light chains. Clone HB57 produces an antibody with one of the highest affinities available (Ka = 5.34 x 108 M-1).1 Antigen Distribution Surface IgM is expressed on B-lymphocytes. PubMed Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. In in vivo mouse studies, clone HB57 refers to the use of transgenic mice expressing the human HLA-B*57:01 allele to model immune responses and drug-induced liver injury, particularly for studying hypersensitivity reactions to drugs like abacavir and flucloxacillin. Key applications and context:
In summary, clone HB57 in mouse studies is a transgenic line expressing HLA-B*57:01, widely used to investigate human-like adaptive immune responses to certain drugs, mechanisms of tolerance, and the cellular basis for HLA-associated hypersensitivity syndromes. There is no specific information available about the storage temperature for a sterile packaged clone named HB57 in the provided search results. However, for general sterile medical supplies, it is recommended that storage temperatures do not exceed 72°F (22°C) to 78°F (26°C) to maintain sterility and integrity. If HB57 is related to a specific biological product or reagent, such as the IgM Antibody (DA4-4 same as SA-DA4 or HB57), it would typically require storage at lower temperatures, such as refrigeration at 4°C, especially if it is a sensitive biological reagent. It's advisable to consult specific product guidelines or manufacturer recommendations for precise storage conditions. There is no direct evidence in the provided search results that identifies specific antibodies or proteins commonly used alongside HB57 in the literature. The available information describes HB57 (clone ID) as a monoclonal antibody specific for the human IgM ?-chain constant region (target: Ig mu chain C region, Ig mu chain C region BOT, IGHM), with applications such as flow cytometry (FCM), Western blot (WB), and immunohistochemistry (IHC). In typical laboratory use, antibodies like HB57 are often paired with reagents that detect different immunoglobulin isotypes (e.g., anti-IgG), secondary antibodies (e.g., anti-mouse IgG for detection of mouse-derived primary antibodies like HB57), or antibodies specific for cellular markers (e.g., CD19 for B cells if studying B cell IgM expression). However, the search results do not cite any specific examples of these pairings or named collaborations of HB57 with other antibodies or proteins in published studies. In summary: The clone HB57, specifically in the form of dextran-conjugated anti-? mAb HB57 (HB57-dex), is referenced in scientific literature primarily for its role in B-cell receptor (BCR) stimulation and its impact on telomerase activity and cell survival in B-cell chronic lymphocytic leukemia (B-CLL) clones. Here are the key findings:
These findings highlight the importance of valency and ligand structure in BCR signaling and its downstream effects on telomerase activity and cell fate in B-CLL clones. References & Citations1. Rudich, S. M. et al. (1988) J. Exp. Med. 168:247
Technical ProtocolsCertificate of Analysis |
Formats Available
Prod No. | Description |
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I-995 | |
I-987 | |
I-988 | |
I-989 | |
I-991 | |
I-992 | |
I-993 | |
I-121 | |
I-1202 | |
I-122 | |
I-123 | |
I-1201 |
