Anti-Human IgM µ-Chain Specific – DyLight® 650

Anti-Human IgM µ-Chain Specific – DyLight® 650

Product No.: I-991

[product_table name="All Top" skus="I-991"]

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Clone
HB57
Target
IgM µ-Chain Specific
Formats AvailableView All
Product Type
Monoclonal Antibody
Isotype
IgG1
Applications
FC

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Product Size
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Antibody Details

Product Details

Reactive Species
Human
Host Species
Mouse
Immunogen
Purified Recombinant Human IgM µ-Chain Specific (>98%)
Product Concentration
0.2 mg/ml
Formulation
This DyLight® 650 conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This DyLight® 650 conjugate is stable when stored at 2-8°C. Do not freeze.
Cross Reactivity
Cross-reactivity by ELISA against human myeloma proteins:
Human IgG1: 100%
Human IgG2: 100%
Human IgG3: 100%
Human IgG4: 100%
Human IgM: <0.2%
Human IgA: <0.1%
Human IgE: <0.1%

There is no detectable binding to bovine, goat, horse sheep IgG.

NOTE: This Anti-Human IgG, Fc Fragment Specific (Clone HB57) when conjugated to HRP is validated for use in Enzyme Immunoassay for the detection of Human IgG, µ-Chain Specific (Part No.: I-1201)
Country of Origin
USA
Shipping
Next Day 2-8°C
Excitation Laser
Red Laser (652 nm)
Applications and Recommended Usage?
Quality Tested by Leinco
FC This antibody has been quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Mouse Anti-Human IgM µ-Chain Specific (Clone HB57) recognizes an epitope on Human IgM µ-Chain Specific. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Antigen Distribution
Surface IgM is expressed on B-lymphocytes.

Antigen Details

References & Citations

1. Rudich, S. M. et al. (1988) J. Exp. Med. 168:247
Flow Cytometry
Products are for research use only. Not for use in diagnostic or therapeutic procedures.