Anti-Human IgM µ-Chain Specific – Purified

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Pricing & Details

Product No.I-121
Clone
HB57
Protein
IgM µ-Chain Specific
Formats AvailableView All
Product Type
Monoclonal Antibody
Isotype
Mouse
IgG1
Applications
ELISA
,
FC
Prod No.
Size
Price
Avail.
Qty
Add to cart
I-121-0.25 mg
0.25 mg
$134.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Mouse
Product Concentration
0.5 mg/ml
Formulation
This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This antibody is stable when stored at 2-8°C. Do not freeze.
Cross Reactivity
Cross-reactivity by ELISA against human myeloma proteins:
Human IgG1: 100%
Human IgG2: 100%
Human IgG3: 100%
Human IgG4: 100%
Human IgM: <0.2%
Human IgA: <0.1%
Human IgE: <0.1%

There is no detectable binding to bovine, goat, horse sheep IgG.

NOTE: This Anti-Human IgG, Fc Fragment Specific (Clone HB57) when conjugated to HRP is validated for use in Enzyme Immunoassay for the detection of Human IgG, µ-Chain Specific (Part No.: I-1201)
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
FC This antibody has been quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Anti-Human IgM (µ chain specific) monoclonal antibody reacts with human IgM through an epitope on the heavy chain. This antibody is non-reactive with IgG, IgA or light chains. Clone HB57 produces an antibody with one of the highest affinities available (Ka = 5.34 x 108 M-1).1
Antigen Distribution
Surface IgM is expressed on B-lymphocytes.

Antigen Details

References & Citations

1. Rudich, S. M. et al. (1988) J. Exp. Med. 168:247
Elisa Sandwich Protocol
Flow Cytometry

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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