Anti-Human IL-11 (Clone 22616)

Pricing & Details

Product No.I-153
Clone
22616
Protein
IL-11
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Interleukin-11, AGIF(Adipogenesis Inhibitory Factor)
Isotype
Mouse
IgG2b
Applications
ELISA Cap
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
I-153-25 µg
25 µg
$180.00
In stock
Max:
Min: 1
Step: 1
I-153-100 µg
100 µg
$430.00
In stock
Max:
Min: 1
Step: 1
I-153-500 µg
500 µg
$935.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Mouse
Immunogen
Purified Recombinant Human IL-11 (Accession # P20809)
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2 - 8 µg/ml. A suitable detection antibody is PN:I-266 at a concentration of approximately 0.1-0.4 µg/ml. A suggested standard for this assay is PN:I-194.
Western Blotting: To detect Human IL-11 this monoclonal antibody can be used at a concentration of 1 - 2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human IL-11 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:I-194.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Mouse Anti-Human Interleukin-11 (IL-11) (Clone 22616) recognizes an epitope on Human IL-11. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody shows 100% cross-reactivity with rmIL-11 and no cross-reactivity with rhIL-6, rhIL-6 R, rhLIF, rhOSM, rhCT-1, rhCNTF, or rhCLC.
Background
Interleukin 11 (IL-11) is a multifunctional cytokine isolated from bone marrow-derived stromal cells. It is a key regulator of multiple events in hemtaopoiesis, most notably the stimulation of megakaryocyte maturation.1 It is also known under the names Adipogenesis inhibitory factor (AGIF)2 and Oprelvekin. IL-11 improves platelet recovery after chemotherapy-induced thrombocytopenia, induces acute phase proteins, modulates antigen-antibody responses, participate in the regulation of bone cell proliferation and differentiation and could be use as a therapeutic for osteoporosis. Besides from lymphopoietic/hematopoietic and osteotrophic properties, it has functions in many tissues such as brain, gut and testis.

Antigen Details

Protein
PubMed

References & Citations

1. Williams, DA. et al. (1990) Proc. Natl. Acad. Sci. USA87: 7512
2. Takiguchi, Y. et al. (1991) FEBS Lett. 283: 199
Elisa Sandwich Protocol
General Western Blot Protocol

Formats Available

Prod No.
Description
I-153
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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