Anti-Human IL-13 Rα1
Anti-Human IL-13 Rα1
Product No.: I-625
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Clone 116730 Target IL-13 R alpha 1 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Interleukin-13 Receptor Alpha 1, IL13RA1, CD213A1, IL-13Ra, NR4 Isotype IgG2b Applications ELISA Cap , WB |
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Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Immunogen Purified Recombinant Human IL-13 Rα1 (Accession # P78552) Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830747 Applications and Recommended Usage? Quality Tested by Leinco ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suggested standard for this assay is PN:I-548. Western Blotting: To detect Human IL-13 Rα1 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human IL-13 Rα1 is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:I-548. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Mouse Anti-Human IL-13 Receptor α1 (IL-13 Rα1) (Clone 116730) recognizes an epitope on Human IL-13 Rα1. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. No cross-reactivity was observed with rmIL-13 Rα1,rhIL-13 Rα2, rhIL-4 R, rhIL-5 Rα, rhIL-5 Rβ, or rhIL-9 R Background Interleukin 13 receptor, alpha 1, also known as IL13RA1 and CD213A1 (cluster of differentiation 213A1) is a subunit of the interleukin 13 receptor. This subunit forms a receptor complex with IL4 receptor alpha, a subunit shared by IL13 and IL4 receptors. This subunit serves as a primary IL13-binding subunit of the IL13 receptor, and may also be a component of IL4 receptors. This protein has been shown to bind tyrosine kinase TYK2, and thus may mediate the signaling processes that lead to the activation of JAK1, STAT3 and STAT6 induced by IL13 and IL4. IL-13Ralpha1is essential for allergen-induced airway hyperreactivity and mucus hypersecretion.1 Antigen DetailsPubMed References & Citations1. Caput, D. et al. (1996) J. Biol. Chem. 271:16921 2. Donaldson, DD. et al. (1998) J. Immunol. 161:2317 3. Hilton, DJ. et al. (1996) Proc. Natl. Acad. Sci. (USA) 93:497 4. Zhang, JG. et al. (1997) J. Biol. Chem. 272:9474 5. Orchansky, PL. et al. (1999) J. Biol. Chem. 274:0818 Technical Protocols  |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
