Anti-Human IL-16 – Biotin

Pricing & Details

Product No.I-271
Protein
IL-16
Formats AvailableView All
Product Type
Polyclonal Antibody
Alternate Names
Lymphocyte Chemoattractant Factor, Interleukin-16
Applications
ELISA Det
,
FC
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
I-271-50 µg
50 µg
$525.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Goat
Immunogen
E. coli-Derived Recombinant Human Interleukin-16 Isoform 1, C-Terminal Peptide (Accession # Q14005)
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.4 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
Storage and Handling
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted biotin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing IL-16. A suggested method would be to stain cells expressing IL-16 with 2.5 µg/106 cells in a total staining volume of 100 µl followed by PN:A104.
Western Blotting: To detect Human IL-16 this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Human IL-16 is 1 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
ELISA Detection: Suitable for use at concentration of 0.1-0.4 µg/mL. Use in combination with: Capture Reagent: Human IL‑16 Antibody. Standard: Recombinant Human IL-16 Protein
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Goat Anti-Human Interleukin-16 (IL-16) recognizes Human IL-16. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background
IL-16 is a CD8+ T cell-derived cytokine that induces chemotaxis of CD4+ T cells and CD4+ monocytes and eosinophils. Analysis by gel filtration suggests that, under physiological conditions, hIL-16 exists predominantly as a noncovalently linked multimer, but that some IL-16 may exist as a monomer. However, only the multimeric form appears to possess chemotactic activity, suggesting that receptor cross-linking may be required for activity. IL-16 also induces expression of IL-2 receptor (IL-2R) and MHC class II molecules on CD4 + T cells. Human and mouse IL-16 show significant cross-species reactivity.

Antigen Details

Protein
PubMed
NCBI Gene Bank ID
Indirect Elisa Protocol
Flow Cytometry
General Western Blot Protocol
Prod No.
Description
I-158
I-271
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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