Anti-Human IL-16

Pricing & Details

Product No.I-158
Protein
IL-16
Formats AvailableView All
Product Type
Polyclonal Antibody
Alternate Names
Interleukin-16, FLJ16806, HsT19289, Lymphocyte Chemoattractant Factor (LCF), PrIL-16
Applications
CyTOF®
,
FC
,
ICC
,
IHC FF
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
I-158-25 µg
25 µg
$185.00
In stock
Max:
Min: 1
Step: 1
I-158-100 µg
100 µg
$442.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Goat
Immunogen
Purified Recombinant Human IL-16 (Accession # Q14005)
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.4 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Storage and Handling
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing IL-16. A suggested method would be to stain cells expressing IL-16 with 0.25 µg per 106 cells in a total staining volume of 100 µl followed by an appropriate secondary antibody.
Western Blotting: To detect Human IL-16 this polyclonal antibody can be used at a concentration of 1 µg/ml. A suitable Western blotting control is PN:T261.
Other Applications Reported In Literature ?
Immunohistochemistry on Frozen Sections: This antibody should give satisfactory staining results when used on frozen sections at a concentration of 5-15 µg/ml.
CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry: Suitable for use at concentration of 5-15 µg/mL.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Goat Anti-Human Interleukin-16 (IL-16) recognizes Human IL-16. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background
Interleukin 16 (lymphocyte chemoattractant factor), also known as IL16, is a pleiotropic cytokine that functions as a chemoattractant, a modulator of T cell activation, and an inhibitor of HIV replication. The signaling process of this cytokine is mediated by CD4. IL-16 is released by a variety of cells (including lymphocytes and some epithelial cells) that has been characterized as a chemoattractant for certain immune cells expressing the cell surface molecule CD4. IL-16 has a critical role in protecting antigen presenting cells (APC) against HIV-1 infection and is used in the treatment of HIV disease.1

Antigen Details

Protein
PubMed
NCBI Gene Bank ID

References & Citations

1. Bahr, GM. et al. (1999) J. Virol. 73: 7008
Flow Cytometry
IHC
General Western Blot Protocol
Prod No.
Description
I-158
I-271
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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