Purified Recombinant Human IL-9 (>98%)
<10 ng/mg as determined by the LAL method
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Storage and Handling
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
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Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Human IL-9 this polyclonal antibody can be used at a concentration of 0.1 - 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Human IL-9 is 2 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:I-379.
Other Applications Reported In Literature ?
Neutralization: This antibody is useful for neutralization of Human IL-9 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human IL-9 (at 5 ng/ml) is 2-5 µg/ml.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Rabbit Anti-Human Interleukin-9 (IL-9) recognizes Human IL-9. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Interleukin 9, also known as IL9, is a TH2 cytokine1 belonging to the group of interleukins. This cytokine stimulates cell proliferation and prevents apoptosis. It functions through the interleukin-9 receptor (IL9R), which activates different signal transducer and activator (STAT) proteins and thus connects this cytokine to various biological processes. IL-9 stimulates the proliferation of a variety of hematopoietic lineages through its interaction with a receptor of the cytokine receptor superfamily.2 IL-9 plays a role in the complex pathogenesis of bronchial hyperresponsiveness as a risk factor for asthma.3
NCBI Gene Bank ID
References & Citations
1. Renauld, JC. et al. (1995) J. Immunol 154(10):5061-70.
2. Yang, YC. et al. (1995) J. Biol. Chem. 270(35):20497-502.
3. Levitt, RC. et al. (1997) Proc Natl Acad Sci USA. 94(24):13175-80.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.