Recombinant Rat IL-9

Recombinant Rat IL-9

Product No.: I-842

[product_table name="All Top" skus="I-842"]

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Alternate Names
Interleukin-9, p40 Cytokine, T-Cell Growth Factor p40
Product Type
Recombinant Protein
Expression Host
NS0 Cells
Species
Rat
Applications
ELISA Cap
FC

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Product Size
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Protein Details

Purity
>90% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.01 EU/µg as determined by the LAL method
Protein Accession No.
Amino Acid Sequence
qrcstswgiq htsylienlk ddpsskcscs anvtsclclp ipsddcttpc fqegmsqvtn atqqskfspf ffrvkrivet lksnkcqffs cekpcnqtta gntvsflksl lktfqktevq vqrsra
N-terminal Sequence Analysis
Gln19
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Rat IL-9 is Mr 14.1 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 30-35 kDa.
Predicted Molecular Mass
14.1
Formulation
This recombinant protein was lyophilized from a 0.2 μm filtered solution in 35% acetonitrile (CH3CN) and 0.1% trifluoroacetic acid (TFA).
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day Ambient
NCBI Gene Bank
Applications and Recommended Usage ?
(Quality Tested by Leinco)
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 5 µg/ml (100 µl/well) µg/ml.
Flow Cytometry: PN:A106
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing CXCR5. A suggested method would be to stain cells expressing CXCR5 with approximately 10 µl per test. A typical test sample constitutes approximately 50 µl of packed whole blood or 1 x 105 continuous passage or activated cell cultures that have been centrifuged at 500 X g for five minutes. Labeling of the cells with the biotin conjugate should be followed by PN:A104, resuspended in 200-400 µl of 1X PBS.
Elisa Sandwich Protocol
Flow Cytometry
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.