Anti-Rat IL-9
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Antibody DetailsProduct DetailsReactive Species Rat Host Species Rat Immunogen Purified Recombinant Rat IL-9 (>98%) Formulation This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830946 Applications and Recommended Usage? Quality Tested by Leinco Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing IL-9. A suggested method would be to stain cells expressing IL-9 with 25 µg per 1-2.5 x 106 cells in a total staining volume of 200 µl followed by PN:R485. Western Blotting: To detect Rat IL-9 this polyclonal antibody can be used at a concentration of 0.1-0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The detection limit for Rat IL-9 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:I-842. Additional Applications Reported In Literature ? Neutralization: This antibody is useful for neutralization of Rat IL-9 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Rat IL-9 (at 10 ng/ml) is 1.5-7.5 µg/ml. CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Rat Anti-Rat IL-9 (IL-9) recognizes Rat IL-9. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Antigen DetailsPubMed UniProt.org Technical ProtocolsCyTOF®  N  |
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I-842 | |
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I-859 | |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
