Anti-Human MIP-4 – Biotin

Pricing & Details

Product No.M199
Protein
MIP-4
Formats AvailableView All
Product Type
Polyclonal Antibody
Alternate Names
Pulmonary and Activation-Regulated Chemokine (PARC), SCYA18, DC-CK1, AMAC-1, DCCK1, CKb7, CCL18
Applications
ELISA Det
,
FC
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
M199-50 µg
50 µg
$439.00
In stock
Max:
Min: 1
Step: 1

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Goat
Immunogen
Purified Recombinant Human MIP-4 (Accession # P55774)
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
Storage and Handling
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted biotin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Applications and Recommended Usage?
Quality Tested by Leinco
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing MIP-4. A suggested method would be to stain cells expressing MIP-4 with 0.25 µg per 1 x 106 cells in a total staining volume of 100 µl followed by PN:A104.
Western Blotting: To detect Human MIP-4 this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Human MIP-4 is 2 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:M130.
ELISA Sandwich Assay: This antibody can be used as the detection antibody in a sandwich ELISA at a concentration of approximately 0.1-0.4 µg/ml when used in conjunction with PN:C1411 as the capture antibody at 1 µg/ml and an optimal second step reagent such as PN:A106 for the detection of Human MIP-4.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Goat Anti-Human Macrophage Inflammatory Protein-4 (MIP-4) recognizes Human MIP-4. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background
Macrophage Inflammatory Protein-4 (MIP-4) produced in E.Coli is a single, non-glycosylated polypeptide that attracts lymphocytes but not monocytes or granulocytes and is involved in B cell migration into B cell follicles in lymph nodes. It attracts naive T lymphocytes toward dendritic cells and activated macrophages in lymph nodes, has chemotactic activity for naive T cells, CD4+ and CD8+ T cells and thus may play a role in both humoral and cell-mediated immunity responses. MIP-4 is expressed at high levels in lung, lymph nodes, placenta, and bone marrow, not expressed by peripheral blood monocytes, and a monocyte-to-macrophage differentiation is a prerequisite for expression.

Antigen Details

Protein
PubMed

References & Citations

1. Nomiyama, H. et al. (1997) J. Biol. Chem. 272: 5846
2. Kucharzik, T. et al. (2005) J. Pathol. 166: 1647
Indirect Elisa Protocol
Flow Cytometry
General Western Blot Protocol
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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