Anti-Human/Mouse BID

Anti-Human/Mouse BID

Product No.: B645

[product_table name="All Top" skus="B645"]

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Target
BID
Product Type
Polyclonal Antibody
Applications
IP
,
WB

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Product Size
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Antibody Details

Product Details

Reactive Species
Human/Mouse
Host Species
Goat
Immunogen
(E. coli-derived)Purified Recombinant Human/Mouse BID (>98%)
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
State of Matter
Lyophilized
Storage and Handling
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Human/Mouse BID this polyclonal antibody can be used at a concentration of 1.0 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Human/Mouse BID is 1.5-3.0 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Simple Western Blotting: Recommended concentration 10 µg/mL.
Additional Applications Reported In Literature ?
Immunoprecipitation: Suitable for use at concentration of 4 µg/106 cells.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Goat Anti-Human/Mouse BID (BID) recognizes Human/Mouse BID. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Antigen Details

PubMed
Immunoprecipitation Protocol
General Western Blot Protocol
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.