Anti-Human/Mouse HDAC1 (Clone 10E2) – Biotin
Anti-Human/Mouse HDAC1 (Clone 10E2) – Biotin
Product No.: H298
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Clone 10E2 Target HDAC1 Formats AvailableView All Product Type Hybridoma Monoclonal Antibody Alternate Names Histone deacetylase 1, Protein deacetylase HDAC1, Protein deacylase HDAC1 Isotype Mouse IgG1 κ Applications IF Microscopy , IF Staining , IP , WB , Chromatin IP (ChIP) |
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Antibody DetailsProduct DetailsReactive Species Human ⋅ Mouse Host Species Mouse Immunogen N/A Product Concentration 0.5 mg/ml Formulation This Biotinylated antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative. State of Matter Liquid Storage and Handling This biotinylated antibody is stable when stored at 2-8°C. Do not freeze. Regulatory Status Research Use Only Country of Origin USA Shipping 2 – 8° C Wet Ice Additional Applications Reported In Literature ? IF microscopy, IF staining, IP, WB Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity 10E2 activity is directed against human and mouse HDAC1. Background HDAC1 is a histone deacetylase that plays an important role in transcriptional regulation, cell
cycle progression, and development1 as well as regulates major endothelial functions such as
angiogenesis, inflammatory signalling, redox homeostasis, and nitric oxide signalling2. As part
of a large multiprotein complex, HDAC1 deacetylates lysine residues on the N-terminal portion
of core histones H2A, H2B, H3 and H41. In this capacity, HDAC1 is involved in chromatin
remodelling, histone acetylation, and RNA polymerase II progression in transcribed regions.
Additionally, HDAC1 deacetylates non-histone targets (NR1D2, RELA, SP1, SP3, STAT3,
RELA, TSHZ3, SP proteins), and thereby regulates their function. HDAC1 is also required for
repression of circadian genes (CLOCK-BMAL-1, PER1, and CRY1). Knockout of HDAC1 in
mice causes impairment to cellular proliferation and death at embryonic day 9.53. Antigen Distribution HDAC1 expression is ubiquitous, with higher levels in heart, pancreas, and
testis tissues. Levels are lower in kidney and brain. HDAC1 primarily localizes to the nucleus. Ligand/Receptor HDAC2, RBBP4, RBBP7, SIN3, SAP18, SAP30 Research Area Cell Biology . Immunology . Neuroscience . Epigenetics References & Citations1 https://www.uniprot.org/uniprotkb/Q13547/entry 2 Dunaway LS, Pollock JS. Cardiovasc Res. 118(8):1885-1903. 2022. 3 Segré CV, Senese S, Loponte S, et al. MAbs. 8(1):37-42. 2016. 4 Hagelkruys A, Lagger S, Krahmer J, et al. Development. 141(3):604-616. 2014. 5 Vcelkova T, Reiter W, Zylka M, et al. Nucleic Acids Res. 51(21):11748-11769. 2023. 6 Torres HM, Fang F, May DG, et al. J Biol Chem. 300(1):105522. 2024. 7 Simões M, Rino J, Pinheiro I, et al. Viruses. 7(9):4978-4996. 2015. 8. Guantes R, Rastrojo A, Neves R, et al. Genome Res. 25(5):633-644. 2015. Technical ProtocolsIF Microscopy IF Staining   Chromatin IP (ChIP) Certificate of Analysis |
Formats Available
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
